Biological distribution of 99mTc-labeled YIGSR and IKVAV laminin peptides in rodents: 99mTc-IKVAV peptide localizes to the lung.
Biological distribution of 99mTc-labeled YIGSR and IKVAV laminin peptides in rodents: 99mTc-IKVAV peptide localizes to the lung.
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DOI：
10.1016/0925-4439(93)90141-m
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发表时间：
1993-09
期刊：
影响因子：
--
通讯作者：
P. Zamora;D. Eshima;D. Graham;L. Shattuck;B. Rhodes
中科院分区：
文献类型：
--
作者：
P. Zamora;D. Eshima;D. Graham;L. Shattuck;B. Rhodes
Two laminin-derived peptides containing either YIGSR or IKVAV (single amino acid code) sequences were radiolabeled with99mTc and their biological distribution evaluated in rodents. Both99mTC-peptides cleared rapidly from the circulation though the kidney, and to a lesser extent, through the liver.99mTC-YIGSR peptide did not accumulate in any organ examined in normal, tumored, and emphysemic mice. The99mTc-IKVAV peptide localized within 10 min to the lung of normal animals, resulting in lung-to-blood ratios of approximately 23:1. The99mTc-IKVAV peptide localized to lung after submicron filtration and after intraperitoneal injection, suggesting that particulates do not play a major role in localization. Pre-incubation of99mTc-IKVAV peptide in whole blood decreased lung localization, suggesting that margination of radiolabeled blood cells does not play a major role in the lung localization. When99mTc-IKVAV was injected into mice with tumored lungs (melanoma), the lung uptake was markedly increased (up to 20% injected dose higher than control lungs) at all time points examined (10, 30, and 120 min). When99mTc-IKVAV was injected into mice with genetic emphysema, the lung uptake was markedly decreased at all time points. The localization of the99mTc-IKVAV-containing peptide to the lung is consistent with a receptor-based mechanism.
