Molecular characterization and expression profiles of GATA6 in tongue sole (Cynoglossus semilaevis)
Molecular characterization and expression profiles of GATA6 in tongue sole (Cynoglossus semilaevis)
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DOI：
10.1016/j.cbpb.2016.03.006
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发表时间：
2016-08-01
影响因子：
2.2
通讯作者：
Wang, Xubo
中科院分区：
文献类型：
--
作者：
Liu, Jinxiang;Zhang, Wei;Wang, Xubo
GATA结合蛋白6（GATA-binding protein 6，GATA 6）是加塔家族的一个转录因子，在性腺细胞增殖、分化和内胚层发育中起重要作用。该序列由1494个核苷酸组成，编码497个氨基酸，包含两个保守的锌指结构域。系统发育、基因结构和共线性分析表明，C.半滑鱼GATA 6与硬骨鱼和四足动物GATA 6同源。C.半滑舌鳎GATA 6 mRNA在心脏、肠、肝脏、肾脏和性腺中有高表达。胚胎发育表达谱显示，GATA 6参与形态发生，因为其表达在囊胚阶段增加。原位杂交结果显示，在睾丸的精原细胞、精母细胞和支持细胞中有较强的GATA 6信号。在卵巢的卵原细胞和卵母细胞中也检测到信号。C.半滑鱼GATA 6是性二态的，并且启动子区域的甲基化模式在雄性、雌性和假单胞菌之间变化。这些结果表明，GATA 6可能影响了中华绒螯蟹的性腺发育和生殖。半滑鱼本研究为进一步发展我国红腹锦鸡的育种技术奠定了基础。半滑鱼(C)版权所有© 2016 Elsevier Inc.
GATA-binding protein 6 (GATA6), a transcription factor of the GATA family, plays an important role in gonadal cell proliferation, differentiation, and endoderm development In this study, the full-length coding sequence of tongue sole (Cynoglossus semilaevis) GATA6 was identified. The sequence consisted of 1494 nucleotides encoding a peptide of 497 amino acids, which included two conserved zinc finger domains. Phylogenetic, gene structure, and synteny analysis showed that C. semilaevis GATA6 was homologous to teleost and tetrapod GATA6. C. semilaevis GATA6 mRNA exhibited high expression in heart, intestine, liver, kidney, and gonad. Embryonic development expression profiles revealed that GATA6 is involved in morphogenesis because its expression increased at the blastula stage. The in situ hybridization results showed strong GATA6 signals in spermatogonia, spermatocytes, and Sertoli cells of the testis. The signals were also detected in the oogonia and oocytes of the ovary. The expression of C. semilaevis GATA6 was sexually dimorphic, and the methylation pattern in the promoter region varied among males, females, and pseudomales. These results suggested that GATA6 might influence the gonad development and reproduction of C. semilaevis. This study provides the groundwork for further development of breeding techniques in C. semilaevis. (C) 2016 Elsevier Inc All rights reserved.