Sequence analysis of env C2/V3, gag p17/p24, and pol protease regions of 25 HIV type 1 isolates from Ho Chi Minh City, Vietnam.
Sequence analysis of env C2/V3, gag p17/p24, and pol protease regions of 25 HIV type 1 isolates from Ho Chi Minh City, Vietnam.
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对越南胡志明市 25 株 HIV 1 型分离株的 env C2/V3、gag p17/p24 和 pol 蛋白酶区域进行序列分析。
DOI：
10.1089/088922201750461357
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发表时间：
2001
影响因子：
1.5
通讯作者：
H. Fleury
中科院分区：
文献类型：
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作者：
A. Caumont;N. T. Lan;N. T. Uyen;P. V. Hung;E. Schvoerer;M. S. Urriza;P. Roques;M. Schrive;T. T. Lien;M. Lafon;D. Dormont;F. Barré;H. Fleury
本文对越南胡志明市25例HIV-1血清学阳性者分离株的Env C2/V3、gag p17/p24、pol蛋白酶和RT区进行了研究，并通过DNA序列分析确定了基因亚型。25个分离株中有23个被鉴定为属于E亚型，现在被认为是循环重组型1（CRF01_AE）。V3环顶部的基序（通常为GPGQ）之前是异亮氨酸或甲硫氨酸（M）残基;与泰国E病毒相比，M残基可能是越南E病毒分离株的局部特征。两个分离株（8%）显示为亚型间重组体：一个E/B和一个CRF 02_AG（IBNG）/D。pol蛋白酶的多态性仅被认为是CRF01_AE分离株，并且明显不同于在位置13、35、36、41、69和89处具有取代的B病毒记录的多态性。
Env C2/V3, gag p17/p24, pol protease, and RT regions of HIV-1 isolates recently obtained from 25 HIV-1 seropositive individuals from Ho Chi Minh City (Vietnam) were studied, and genes subtypes were determined by DNA sequence analyses. Twenty-three isolates out of 25 were identified as belonging to subtype E, now recognized as circulating recombinant form 1 (CRF01_AE). The motif at the top of the V3 loop (generally GPGQ) was then preceded by an isoleucine or a methionine (M) residue; the M residue might be a local signature of Vietnamese E isolates compared to Thai E viruses. Two isolates (8%) were shown to be intersubtype recombinants: one E/B and one CRF02_AG(IBNG)/D. The polymorphism of pol protease was considered only for CRF01_AE isolates and is clearly different from that recorded for B viruses with substitutions at positions 13, 35, 36, 41, 69, and 89.
越南 HIV-1 感染的序列和系统发育分析：商业性工作者 (CSW) 和注射吸毒者 (IDU) 中的 E 亚型。
DOI：
--
发表时间：
1997
期刊：
Cellular and molecular biology (Noisy-le-Grand, France)
影响因子：
--
作者：
Nerurkar,VR;Nguyen,HT;Woodward,CL;Hoffmann,PR;Dashwood,WM;Long,HT;Morens,DM;Detels,R;Yanagihara,R
通讯作者：
Yanagihara,R