TRPC1: a core component of store-operated calcium channels
TRPC1: a core component of store-operated calcium channels
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DOI：
10.1042/bst0350096
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发表时间：
2007-02-01
影响因子：
3.9
通讯作者：
Ambudkar, I. S.
中科院分区：
文献类型：
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作者：
Ambudkar, I. S.
TRPC（瞬时受体电位典型）蛋白响应于激动剂刺激的PIP 2（磷脂酰肌醇4，5-二磷酸）水解而被激活，并且已被建议作为难以捉摸的SOC（钙库操纵的钙通道）的候选组分。TRFC 1是目前SOC中最强的候选成分。在几种不同的细胞类型中，内源性TRPC 1已被证明有助于SOCE（钙库操纵的钙离子进入）。然而，TRFC 1的调控机制及其确切的生理功能尚未建立。我们实验室和其他几个实验室的研究表明，TRPC 1与功能特异性质膜微区中的关键钙信号蛋白组装在信号复合物中。此外，TRPC 1单体之间的关键相互作用以及TRPC 1与其他蛋白质之间的相互作用决定了含TRFC 1通道的表面表达和功能。最近的研究揭示了含TRFC 1的SOC的新型调节剂，并证明了调节CRAC（钙释放激活钙）和SOC通道的共同分子基础。在本文中，我们将重新审视TRPC 1在SOCE中的作用，并讨论TRPC 1的研究如何为验证SOCE的机制提供实验基础。
The TRPC (transient receptor potential canonical) proteins are activated in response to agonist-stimulated PIP2 (phosphatidylinositol 4,5-bisphosphate) hydrolysis and have been suggested as candidate components of the elusive SOC (store-operated calcium channel). TRFC1 is currently the strongest candidate component of SOC. Enclogenous TRPC1 has been shown to contribute to SOCE (store-operated calcium entry) in several different cell types. However, the mechanisms involved in the regulation of TRFC1 and its exact physiological function have yet to be established. Studies from our laboratory and several others have demonstrated that TRPC1 is assembled in a signalling complex with key calcium signalling proteins in functionally specific plasma membrane microdomains. Furthermore, critical interactions between TRPC1 monomers as well as interactions between TRPC1 and other proteins determine the surface expression and function of TRFC1-containing channels. Recent studies have revealed novel regulators of TRFC1 -containing SOCs and have demonstrated a common molecular basis for the regulation of CRAC (calcium-release-activated calcium) and SOC channels. In the present paper, we will revisit the role of TRPC1 in SOCE and discuss how studies with TRPC1 provide an experimental basis for validating the mechanism of SOCE.