Protective Effects and Mechanisms of Shenhua Tablet on Toll-Like Receptors in Rat Model of Renal Ischemia-Reperfusion Injury
Protective Effects and Mechanisms of Shenhua Tablet on Toll-Like Receptors in Rat Model of Renal Ischemia-Reperfusion Injury
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DOI：
10.1007/s11655-017-2756-6
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发表时间：
2019-01-01
影响因子：
2.9
通讯作者：
Chen Xiang-mei
中科院分区：
文献类型：
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作者：
Li Qing-ping;Wei Ri-bao;Chen Xiang-mei
目的：探讨肾化片对大鼠肾缺血再灌注损伤(IRI)后Toll样受体(Toll-like Receptor，TLRs)介导的信号转导通路的保护作用及其可能机制。方法：雄性Wistar大鼠60只，随机分为5组：假手术组、模型对照组、黄芪甲苷150 mg·kg~(-1)·d~(-1)组、SHT1.5和3.0g·kg~(-1)·d~(-1)组。药物治疗一周后，大鼠接受手术建立IRI模型。分别于造模后24 h、72 h测定血清肌酐(Scr)、血尿素氮(BUN)，高碘酸-席夫染色后进行病理损伤评分。免疫组织化学、Western印迹和定量聚合酶链式反应检测TLR2、TLR4和髓系分化因子88(MyD88)蛋白和mRNA的表达。采用酶联免疫吸附试验检测肿瘤坏死因子-α(TNF-α)和白介素6(IL-6)蛋白表达。结果：与假手术组比较，模型组大鼠肾功能改变明显(Scr：189.42+/-21.5，P
Objective: To investigate the protective effects and potential mechanisms of Shenhua Tablet (SHT) on the toll-like receptors (TLRs)-mediated signaling pathways in a rat model of kidney ischemia-reperfusion injury (IRI). Methods: Sixty male Wistar rats were randomly divided into 5 groups: sham surgery, model control, astragaloside (150 mg.kg(-1).d(-1)), low-and high-dose SHT (1.5 and 3.0 g.kg(-1).d(-1), repectively) groups. One week after drug treatment, rats underwent surgery to establish the IRI models. At 24 h and 72 h after the modeling, serum creatinine (Scr) and blood urea nitrogen (BUN) were analyzed; pathological damage were scored after periodic acid-Schiff staining. TLR2, TLR4 and myeloid differentiation factor 88 (MyD88) protein and mRNA expressions were detected by inmmunohistochemistry, Western blot and qPCR. Tumor necrosis factor alpha (TNF-alpha) and interleukin-6 (IL-6) protein expressions were detected by enzyme linked immunosorbent assay. Results: Compared with the sham group, the model group exhibited severe change in renal function (Scr: 189.42 +/- 21.50, P