Root endophytic fungi show low levels of interspecific competition in planta
Root endophytic fungi show low levels of interspecific competition in planta
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DOI：
10.1016/j.funeco.2019.02.009
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发表时间：
2019-06-01
期刊：
影响因子：
2.9
通讯作者：
Macia-Vicente, Jose G.
中科院分区：
文献类型：
--
作者：
Kia, Sevda Haghi;Pallesch, Sascha;Macia-Vicente, Jose G.
根与影响植物生长和健康的真菌群落有关。单个根相关真菌对植物性能有不同的影响，从有害到有益，但几乎不知道它们的种间相互作用如何决定植物适应性。在此，我们评估了植物中具有不同程度系统发育和性状相似性的优势根定殖真菌之间的相互作用，以及它们共同存在对各自定殖根能力的影响及其对植物生长的影响。以拟南芥为寄主植物，与真菌菌株单独或成对组合共培养，进行体外生物测定。利用实时定量PCR技术监测菌株的根定殖情况，并通过测定植株生物量估算菌株对寄主生长的影响。菌株对植物生长有不同的影响，尽管这些影响大多是适度的，并且很少受到其他真菌存在的影响。根内真菌丰度对共接种的响应不同，但菌株间的竞争与它们在功能性状上的相似性无关。我们的研究结果表明，优势真菌根内生菌之间几乎没有竞争，这表明它们占据互补的根生态位，这可以解释在自然条件下健康寄主的高度真菌多样性。(C) 2019 Elsevier Ltd和英国真菌学学会。版权所有。
Roots are associated with fungal communities that affect plant growth and health. Individual root-associated fungi have different effects on plant performance, from detrimental to beneficial, but it is barely known how their inter-species interactions determine plant fitness. Here, we evaluate in planta interactions among dominant root-colonizing fungi with different degrees of phylogenetic and trait similarity, and the impact of their co-occurrence on their respective ability to colonize roots and their effects on plant growth. An in vitro bioassay with Arabidopsis thaliana as host plant was used for the co-cultivation with individual or paired combinations of fungal strains. Root colonization by strains was monitored using real-time quantitative PCR, and the effects on their host's growth were estimated by measuring plant biomass. Strains had variable effects on plant growth, although these effects were mostly modest and little affected by the presence of other fungi. Abundance of each fungus in roots responded differently to co-inoculation, but competition between strains was not associated with their similarity in functional traits. Our findings show little competition between dominant fungal root endophytes, which suggests that they occupy complementary root niches and could explain the high fungal diversity colonizing healthy hosts in natural conditions. (C) 2019 Elsevier Ltd and British Mycological Society. All rights reserved.