Bisphenol S Interacts with Catalase and Induces Oxidative Stress in Mouse Liver and Renal Cells.
Bisphenol S Interacts with Catalase and Induces Oxidative Stress in Mouse Liver and Renal Cells.
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DOI：
10.1021/acs.jafc.6b02656
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发表时间：
2016-08
影响因子：
6.1
通讯作者：
Rui Zhang;Rutao Liu;Wansong Zong
中科院分区：
文献类型：
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作者：
Rui Zhang;Rutao Liu;Wansong Zong
双酚S（BPS）存在于许多消费品中，并在食品和水中检测到。它也是食品包装工业中双酚A（BPA）的主要替代品。然而，BPS的毒性尚未完全了解。本研究分两部分对BPS的毒性进行研究。首先，氧化应激，细胞活力，凋亡水平，和过氧化氢酶（CAT）活性在小鼠肝细胞和肾细胞BPS暴露后进行了研究。BPS作用12 h后，肝细胞和肾细胞的上述参数在0.1 ~ 1 mM范围内变化均>15%。其次，通过多光谱方法和分子对接研究，在分子水平上研究了BPS与CAT的直接相互作用。BPS通过与CAT底物通道上的Gly 117残基结合，改变CAT的结构和活性。结合力主要为氢键和疏水力。
Bisphenol S (BPS) is present in multitudinous consumer products and detected in both food and water. It also has been a main substitute for bisphenol A (BPA) in the food-packaging industry. Yet, the toxicity of BPS is not fully understood. The present study of the toxicity of BPS was divided into two parts. First, oxidative stress, cell viability, apoptosis level, and catalase (CAT) activity in mouse hepatocytes and renal cells were investigated after BPS exposure. After 12 h of incubation with BPS, all of these parameters of hepatocytes and renal cells changed by >15% as the concentration of BPS ranged from 0.1 to 1 mM. Second, the direct interaction between BPS and CAT on the molecule level was investigated by multiple spectral methods and molecular docking investigations. BPS changed the structure and the activity of CAT through binding to the Gly 117 residue on the substrate channel of the enzyme. The main binding forces were hydrogen bond and hydrophobic force.