Invadopodia Detection and Gelatin Degradation Assay.
Invadopodia Detection and Gelatin Degradation Assay.
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DOI：
10.21769/bioprotoc.997
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发表时间：
2013-12-20
期刊：
影响因子：
0.8
通讯作者：
Diaz, Begona
中科院分区：
文献类型：
--
作者：
Diaz, Begona
This protocol is designed to quantify invadopodia formation and activity. Invadopodia are protrusive structures elaborated by cancer cells that mediate cell attachment and remodeling of the extracellular matrix. These structures contribute to the ability of cancer cells to invade and metastasize. In this protocol, both the presence of invadopodia and their activity is simultaneously assessed and quantified by a fluorescent microscopy-based assay.
