Duck RIG-I CARD Domain Induces the Chicken IFN-β by Activating NF-κB.
Duck RIG-I CARD Domain Induces the Chicken IFN-β by Activating NF-κB.
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Duck Rig-I卡结构域通过激活NF-κB诱导鸡IFN-β。
DOI：
10.1155/2015/348792
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发表时间：
2015
影响因子：
--
通讯作者：
Chen G
中科院分区：
文献类型：
--
作者：
Chen Y;Huang Z;Wang B;Yu Q;Liu R;Xu Q;Chang G;Ding J;Chen G
视黄酸诱导基因I- (RIG-I-)样受体（RLRs）最近被鉴定为病毒RNA的细胞质传感器。rig - 1是RLRs家族的一员，在先天免疫中发挥重要作用。虽然先前的研究已经证明鸡中不存在RIG-I，但鸡是否能对RIG-I配体产生反应仍然是一个很大的未知数。本研究成功构建了编码duRIG-I全长（鸭RIG-I，包含所有结构域）、duRIG-I n -末端（包含两个caspase激活和募集结构域，CARDs）和duRIG-I c -末端（包含解旋酶和调控结构域）的真核表达载体，并通过western blotting进行了检测。荧光素酶报告基因法和酶联免疫吸附法（ELISA）检测发现，当多肌苷-多胞酸（poly[I:C]，合成双链RNA）攻毒鸡胚成纤维细胞（DF1细胞）时，durig - 1显著激活NF-κB，诱导IFN-β的表达，而在无poly[I:C]时，durig - 1无活性。进一步的分析表明，无论poly是否存在，CARDs （duRIG-I- n）都能诱导IFN-β的产生[I:C]，而缺乏card的duRIG-I （duRIG-I-C）不能激活下游信号。这些结果表明durig - 1 CARD结构域在诱导IFN-β中发挥重要作用，为进一步研究rig - 1在禽先天免疫中的功能提供了基础。
Retinoic acid-inducible gene I- (RIG-I-) like receptors (RLRs) have recently been identified as cytoplasmic sensors for viral RNA. RIG-I, a member of RLRs family, plays an important role in innate immunity. Although previous investigations have proved that RIG-I is absent in chickens, it remains largely unknown whether the chicken can respond to RIG-I ligand. In this study, the eukaryotic expression vectors encoding duRIG-I full length (duck RIG-I, containing all domains), duRIG-I N-terminal (containing the two caspase activation and recruitment domain, CARDs), and duRIG-I C-terminal (containing helicase and regulatory domains) labeled with 6∗His tags were constructed successfully and detected by western blotting. Luciferase reporter assay and enzyme-linked immunosorbent assay (ELISA) detected the duRIG-I significantly activated NF-κB and induced the expression of IFN-β when polyinosinic-polycytidylic acid (poly[I:C], synthetic double-stranded RNA) challenges chicken embryonic fibroblasts cells (DF1 cells), while the duRIG-I was inactive in the absence of poly[I:C]. Further analysis revealed that the CARDs (duRIG-I-N) induced IFN-β production regardless of the presence of poly[I:C], while the CARD-lacking duRIG-I (duRIG-I-C) was not capable of activating downstream signals. These results indicate that duRIG-I CARD domain plays an important role in the induction of IFN-β and provide a basis for further studying the function of RIG-I in avian innate immunity.
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影响因子：
3.7
作者：
Lemos de Matos A;McFadden G;Esteves PJ
通讯作者：
Esteves PJ
影响因子：
2.9
作者：
Chen, Yang;Zhang, Yang;Chen, Guohong
通讯作者：
Chen, Guohong
IRF-5和NF-κBP50在TLR9刺激人浆细胞类树突状细胞中共调节IFN-β和IL-6表达。
影响因子：
5.4
作者：
Steinhagen F;McFarland AP;Rodriguez LG;Tewary P;Jarret A;Savan R;Klinman DM
通讯作者：
Klinman DM
影响因子：
3
作者：
Duan, Y;Wu, C;Kollman, P
通讯作者：
Kollman, P
DOI：
10.1073/pnas.0606699104
发表时间：
2007-01-09
影响因子：
11.1
作者：
Saito, Takeshi;Hirai, Reiko;Gale, Michael, Jr.
通讯作者：
Gale, Michael, Jr.