“Sickle cell anemia: tracking down a mutation”: an interactive learning laboratory that communicates basic principles of genetics and cellular biology
“Sickle cell anemia: tracking down a mutation”: an interactive learning laboratory that communicates basic principles of genetics and cellular biology
复制标题
– 镰状细胞性贫血：追踪突变 –：一个互动学习实验室，传达遗传学和细胞生物学的基本原理
DOI：
10.1152/advan.00143.2015
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发表时间：
2016
影响因子：
2.1
通讯作者：
Wyss, J. Michael
中科院分区：
文献类型：
--
作者：
Jarrett, Kevin;Williams, Mary;Horn, Spencer;Radford, David;Wyss, J. Michael
“镰状细胞性贫血：追踪突变”是一个全天的，以探究为基础的生物学体验，适用于就读遗传学或高级生物学课程的高中生。在经验中，学生使用限制性内切酶消化，醋酸纤维素凝胶电泳和显微镜检查，以发现三个假定的患者中的哪一个具有镰状细胞基因型/表型，使用携带镰状细胞突变的野生型和转基因小鼠的DNA和血液样本。以探究为基础的解决问题的方法促进了学生对遗传学和细胞和分子生物学基本概念的理解，并提供了使用当代生物技术工具的经验。它还使学生了解这种遗传疾病的原因和后果，这种疾病在非洲人后裔中相对常见，并增加了他们对遗传学基本原则的理解。该协议提供了最佳的学习时，由训练有素的主持人（包括课堂教师），并在小组（6：1的学生与教师的比例）进行。这种高质量的体验可以以相对较低的成本提供给大量学生，与当地科学博物馆和/或大学合作尤其有效。在过去的15年中，超过12，000名学生完成了这种基于探究的学习体验，并在他们对疾病和遗传学的理解方面表现出一致的大幅增长。
“Sickle cell anemia: tracking down a mutation” is a full-day, inquiry-based, biology experience for high school students enrolled in genetics or advanced biology courses. In the experience, students use restriction endonuclease digestion, cellulose acetate gel electrophoresis, and microscopy to discover which of three putative patients have the sickle cell genotype/phenotype using DNA and blood samples from wild-type and transgenic mice that carry a sickle cell mutation. The inquiry-based, problem-solving approach facilitates the students' understanding of the basic concepts of genetics and cellular and molecular biology and provides experience with contemporary tools of biotechnology. It also leads to students' appreciation of the causes and consequences of this genetic disease, which is relatively common in individuals of African descent, and increases their understanding of the first principles of genetics. This protocol provides optimal learning when led by well-trained facilitators (including the classroom teacher) and carried out in small groups (6:1 student-to-teacher ratio). This high-quality experience can be offered to a large number of students at a relatively low cost, and it is especially effective in collaboration with a local science museum and/or university. Over the past 15 yr, >12,000 students have completed this inquiry-based learning experience and demonstrated a consistent, substantial increase in their understanding of the disease and genetics in general.