A Multi-Tissue Multi-Omics Analysis Reveals Distinct Kinetics in Entrainment of Diurnal Transcriptomes by Inverted Feeding
A Multi-Tissue Multi-Omics Analysis Reveals Distinct Kinetics in Entrainment of Diurnal Transcriptomes by Inverted Feeding
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DOI：
10.2139/ssrn.3782778
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发表时间：
2021-02
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通讯作者：
Haoran Xin;Fang Deng;Meiyu Zhou;Rongfeng Huang;Xiaogen Ma;He Tian;Yan Tan;Xinghua Chen;D. Deng;G. Shui;Zhihui Zhang;Min-Dian Li
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作者：
Haoran Xin;Fang Deng;Meiyu Zhou;Rongfeng Huang;Xiaogen Ma;He Tian;Yan Tan;Xinghua Chen;D. Deng;G. Shui;Zhihui Zhang;Min-Dian Li
进食的时间与新陈代谢和生理的昼夜节律有关。倒置喂养逆转外周生物钟，但特权位于视交叉上核的光响应中央时钟。然而，在外周组织中，昼夜节律代谢和生理学的全系统变化夹带到倒置喂养仍然在很大程度上未被探索。在这里，我们进行了24小时的全球概况的转录物和代谢物在小鼠外周组织中研究的转换动力学在倒置喂养，并揭示了不同的动力学相夹带昼夜转录组倒置喂养，从脂肪组织（几乎完全夹带），肝脏，肾脏，心脏分级。组织时钟的相动力学与转录组的相动力学一起跟踪，并通过光线索进行门控。转录物和代谢产物的综合分析表明，尽管心脏时钟的动力学缓慢，但脂肪酸氧化完全参与心脏的反向进食。这种多组学资源定义了外周组织中反向进食的昼夜节律特征（www.CircaMetDB.org.cn）。
Time of eating synchronizes circadian rhythms of metabolism and physiology. Inverted feeding reverses peripheral circadian clocks, but privileges the light-responsive central clock located in the suprachiasmatic nucleus. However, system-wide changes of circadian metabolism and physiology entrained to inverted feeding in peripheral tissues remain largely unexplored. Here, we performed a 24-h global profiling of transcripts and metabolites in mouse peripheral tissues to study the transition kinetics during inverted feeding, and revealed distinct kinetics in phase entrainment of diurnal transcriptomes by inverted feeding, which graded from fat tissue (near-completely entrained), liver, kidney, to heart. Phase kinetics of tissue clocks tracked with those of transcriptomes, and was gated by light cue. Integrated analysis of transcripts and metabolites demonstrated that fatty acid oxidation entrained completely to inverted feeding in heart despite the slow kinetics of the heart clock. This multi-omics resource defines circadian signatures of inverted feeding in peripheral tissues (www.CircaMetDB.org.cn).