Ferulic acid promotes endothelial cells proliferation through up-regulating cyclin D1 and VEGF
Ferulic acid promotes endothelial cells proliferation through up-regulating cyclin D1 and VEGF
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DOI：
10.1016/j.jep.2011.07.019
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发表时间：
2011-09-02
影响因子：
5.4
通讯作者：
Zhang, Junping
中科院分区：
文献类型：
--
作者：
Wang, Jun;Yuan, Zhuo;Zhang, Junping
民族药理学相关性：阿魏酸（Ferulic Acid，FA）是一种常用的中草药，具有神经保护、促进造血、治疗肿瘤等作用。先前的研究已经证明，黄芪能够诱导体内血管生成。本研究探讨了FA对人脐静脉内皮细胞系（ECV 304）增殖的影响，探讨了FA对内皮细胞增殖的调节作用，探讨了FA对血管生成的关键环节--内皮细胞的调节作用。一系列的试验被用来检测FA对：（i）细胞增殖;（ii）DNA合成;（iii）细胞周期分布;和（iv）细胞周期蛋白D1和血管内皮生长因子（VEGF）的mRNA表达的影响。这些进行了评估，使用细胞计数试剂盒-8（CCK-8），溴脱氧尿苷酶联免疫吸附试验（BrdU-ELISA），流式细胞术和逆转录聚合酶链反应（RT-PCR），分别。结果和讨论：结果表明，FA在浓度范围从0.1 μ g/mL至10 μ g/mL可以显着提高细胞增殖和DNA合成的剂量依赖性方式。流式细胞术显示G（0）/G（1）期细胞百分比显著减少，S期细胞百分比显著增加。结论：FA能促进ECV 304细胞的增殖。这种作用可能是通过调节细胞周期蛋白D1和血管内皮生长因子来实现的。(C)2011爱思唯尔爱尔兰有限公司保留所有权利。
Ethnopharmacological relevance: Angelicae Sinensis is a well known and commonly used traditional Chinese herbal medicine with many therapeutic effects such as neuroprotection, the promotion of hematopoiesis and the treatment of tumors.Aim of the study: Ferulic acid (FA) is the main active component in Angelicae Sinensis. Previous research has demonstrated that Angelicae Sinensis is able to induce angiogenesis in vivo. This study investigated the effects of FA on the proliferation of a human umbilical vein endothelial cell line (ECV304) with regard to the modulation of endothelial cells, which is a key step of angiogenesis.Materials and methods: ECV304 cells were incubated with FA at different dosages (0.1 mu g/mL, 1 mu g/mL and 10 mu g/mL). A series of assays was used to detect the effects of FA on: (i) cell proliferation; (ii) DNA synthesis; (iii) cell-cycle distribution; and (iv) mRNA expression of cyclin D1 and vascular endothelial growth factor (VEGF). These were assessed using the Cell Counting Kit-8 (CCK-8), bromodeoxyuridine-enzyme-linked immunosorbent assay (BrdU-ELISA), flow cytometry and reverse transcription-polymerase chain reaction (RT-PCR), respectively.Results and discussion: The results showed that FA at a range of concentrations from 0.1 mu g/mL to 10 mu g/mL could markedly improve cell proliferation and DNA synthesis in a dose-dependent manner. Flow cytometry showed a significant decrease in the percentage of cells in the G(0)/G(1) phase and a significant increase in the percentage of cells in the S phase. Furthermore, we found that FA enhanced cyclin D1 and VEGF mRNA expression in ECV304 cells.Conclusion: FA was able to promote ECV304 cells proliferation in vitro. This effect might be observed through the modulation of cyclin D1 and VEGF. (C) 2011 Elsevier Ireland Ltd. All rights reserved.