Smartphone-based integrated voltammetry system for simultaneous detection of ascorbic acid, dopamine, and uric acid with graphene and gold nanoparticles modified screen-printed electrodes
Smartphone-based integrated voltammetry system for simultaneous detection of ascorbic acid, dopamine, and uric acid with graphene and gold nanoparticles modified screen-printed electrodes
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基于智能手机的集成伏安系统，使用石墨烯和金纳米粒子修饰的丝网印刷电极同时检测抗坏血酸、多巴胺和尿酸
DOI：
10.1016/j.bios.2018.07.074
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发表时间：
2018-11-15
影响因子：
12.6
通讯作者：
Liu, Qingjun
中科院分区：
文献类型：
--
作者：
Ji, Daizong;Liu, Zixiang;Liu, Qingjun
抗坏血酸、多巴胺和尿酸是用于健康监测的重要电活性生物分子，它们共存于血清或尿液中。用电化学方法对它们进行定量测定，可为疾病的诊断和治疗提供准确的参考依据。然而，传统的电化学工作站对于现场检测来说太大了。因此，用于检测生物分子的手持式电化学系统的设计对于即时检测（POCT）具有重要意义。本文提出了一种基于智能手机的修饰电极集成伏安法系统，用于生物分子的同时检测。该系统包含一个一次性传感器，一个硬币大小的探测器，和一个配备应用程序的智能手机。丝网印刷电极被用作检测的传感器，在其上通过该系统电化学沉积还原的氧化石墨烯和金纳米颗粒。检测器与伏安法一起使用，其中在传感器上施加激发电压并检测随后的电流响应。智能手机是与检测器通信、计算数据和实时绘制伏安图的核心组件。以生物分子及其混合物的标准溶液为例进行了检测。结果表明，每种物质的峰电流随浓度的增加而增加，该方法可以区分所研究物质的不同电位。最后，通过对人工尿液中生物分子的检测，验证了该系统的实际应用。结果表明，该系统可用于检测生物分子的电化学活性，具有线性，高灵敏度和特定的响应点的护理测试。
Ascorbic acid, dopamine, and uric acid are important electroactive biomolecules for health monitoring and they coexist in serum or urine. Their quantitative determination by electrochemistry could provide the accurate reference for diseases diagnosis and treatment. However, the traditional electrochemical workstations are too large for on-field inspection. Hence, the design of handheld electrochemical system for the detection of biomolecules is significant for point-of-care testing (POCT). In this paper, a smartphone-based integrated voltammetry system using modified electrode was developed for simultaneous detection of biomolecules. The system contained a disposable sensor, a coin-size detector, and a smartphone equipped with application program. Screen-printed electrodes were used as sensors for the detection, on which reduced graphene oxide and gold nanoparticles were electrochemically deposited by the system. The detector was used with voltammetric methods, in which excitation voltage was applied on the sensors and subsequent current responses were detected. The smartphone is the core component to communicate with the detector, calculate data, and plot voltammograms in real-time. Then, the system was applied to detect standard solutions of the biomolecules and their mixtures as examples. The results showed that the peak currents of each substance increased with higher concentration and the method allowed the discrimination of the different potentials of the studied species. Finally, the practical applications of the system were tested through detections of the biomolecules in artificial urine. The results exhibited that the system could be used to detect electrochemical activity of biomolecules with linear, high sensitivity, and specific responses in point-of-care testing.