Cardiac NGF and GDNF expression during Trypanosoma cruzi infection in rats
Cardiac NGF and GDNF expression during Trypanosoma cruzi infection in rats
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DOI：
10.1016/j.autneu.2006.05.004
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发表时间：
2006-12-30
影响因子：
2.7
通讯作者：
da Silva Machado, Conceicao Ribeiro
中科院分区：
文献类型：
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作者：
Martinelli, Patricia Massara;da Silva Camargos, Elizabeth Ribeiro;da Silva Machado, Conceicao Ribeiro
在大鼠中，自主神经末梢在克氏锥虫引起的心肌炎中受损。急性期后逐渐恢复。本研究显示了大鼠感染T - cruzi病毒时，神经胶质细胞系来源的神经营养因子（GDNF）和神经生长因子（NGF）在心脏的水平及其细胞来源。在接种后第20天，即最大交感神经去神经时间点，心房和心室NGF水平（ELISA）显著升高。ELISA未显示心肌GDNF水平明显升高。免疫组织化学显示抗gdnf金颗粒在第20天明显高于心房颗粒。光镜下心房心肌细胞和几根毛细血管显示较强的NGF免疫染色。原位杂交显示，感染和未感染动物心房和心室肌细胞中均存在NGF和GDNF mrna。内皮细胞仅在感染大鼠中表现出NGF mRNA和蛋白。未发现浸润性单核细胞表达神经营养因子的证据。本文首次报道了T - cruzi感染时神经营养因子的表达。我们的研究结果表明，NGF在能够损伤交感神经末梢的心肌炎后的再生现象中发挥重要作用，并保留了前末梢和神经干。GDNF可能有一个较小的或更短暂的参与。(c) 2006 Elsevier B.V.版权所有
In rats, autonomic nerve endings are damaged during Trypanosoma cruzi-induced myocarditis. Gradual recovery occurs after the acute phase. The present work shows the cardiac levels of glial cell line-derived neurotrophic factor (GDNF) and nerve growth factor (NGF), and their cellular sources during T cruzi infection in rats. Atrial and ventricular NGF levels (ELISA) increased significantly at day 20 post inoculation, the time-point of maximal sympathetic denervation. ELISA failed to show significant increase of cardiac GDNF levels. However immunohistochernistry showed a significant increase of anti-GDNF gold particles over atrial granules at day 20. Light microscopy showed stronger NGF immunostaining in atrial cardiomyocytes and several blood capillaries. In situ hybridization showed NGF and GDNF mRNAs in atrial and ventricular myocytes of both infected and uninfected animals. Endothelial cells exhibited NGF mRNA and protein only in infected rats. No evidence of neurotrophic factor expression by the infiltrating mononuclear cells was found. This is the first report on neurotrophic factor expression during T cruzi infection. Our findings indicate an important role for NGF in the regenerative phenomena subsequent to a myocarditis able to damage sympathetic nerve endings, with preservation of preterminals and nerve trunks. GDNF could have a minor or a more transient participation. (c) 2006 Elsevier B.V. All rights reserved.