Role of adiponectin in regulating ovarian theca and granulosa cell function
Role of adiponectin in regulating ovarian theca and granulosa cell function
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DOI：
10.1016/j.mce.2008.01.007
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发表时间：
2008-03-12
影响因子：
4.1
通讯作者：
Spicer, Leon J.
中科院分区：
文献类型：
--
作者：
Lagaly, Dana V.;Aad, Pauline Y.;Spicer, Leon J.
脂联素是一种脂肪因子，与胰岛素抵抗有关，胰岛素抵抗是一种与人类多囊卵巢综合征相关的疾病，但脂联素是否能直接影响卵巢卵泡膜或颗粒细胞功能尚不清楚。因此，为了确定脂联素对大卵泡膜细胞和颗粒细胞的增殖、类固醇生成和基因表达的影响，使用牛卵巢细胞培养物进行了实验。采用RT-PCR方法研究脂联素对卵泡膜细胞和颗粒细胞CYP 11 A1和LH受体（LHR）基因表达的影响，以及对卵泡膜细胞CYP 17 A1和颗粒细胞CYP 19 A1基因表达的影响。脂联素降低（P
Adiponectin is an adipokine that has been implicated in insulin resistance, a condition associated with polycystic ovarian syndrome in humans, but whether adiponectin can directly affect ovarian theca or granulosa cell function is unknown. Therefore, to determine the effects of adiponectin on proliferation, steroidoggenesis and gene expression of large-follicle theca and granulosa cells, experiments were conducted using bovine ovarian cell cultures. RT-PCR was used to elucidate the effects of adiponectin on gene expression of CYP11A1 and LH receptor (LHR) in large-follicle theca and granulosa cells, as well as expression of CYP17A1 in theca cells and CYP19A1 in granulosa cells. Adiponectin decreased (P