In vitro display evolution of unnatural peptides spontaneously cyclized via intramolecular nucleophilic aromatic substitutions
In vitro display evolution of unnatural peptides spontaneously cyclized via intramolecular nucleophilic aromatic substitutions
复制标题
通过分子内亲核芳香取代自发环化非天然肽的体外展示进化
DOI：
10.1039/d2cc00584k
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发表时间：
2022
影响因子：
4.9
通讯作者：
Kawakami Takashi
中科院分区：
文献类型：
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作者：
Yokoyama Takumi;Ando Takehiro;Takamori Yukio;Fuji Daisuke;Sato Masashi;Vedi Santhana;Yamamoto Mizuki;Kawakami Takashi
我们报道了一种新颖的、核糖体可结合的、分子内半胱氨酸反应性氟苯甲酸衍生的用于mrna SELEX的非天然肽连接物，这些非天然肽通过分子内亲核芳香族取代形成硫醚而自发环化。通过这种策略，我们鉴定了几种新的pcsk9结合肽。
We report novel, ribosomally incorporatable, and intramolecularly cysteine-reactive fluorobenzoic acid-derived linkers for SELEX of mRNA-displayed unnatural peptides, which spontaneously cyclized via intramolecular nucleophilic aromatic substitutions forming thioethers. With this strategy we identified several novel PCSK9-binding peptides.