Subtypes of glial cells in the Drosophila embryonic ventral nerve cord as related to lineage and gene expression
Subtypes of glial cells in the Drosophila embryonic ventral nerve cord as related to lineage and gene expression
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DOI：
10.1016/j.mod.2007.12.004
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发表时间：
2008-05-01
影响因子：
2.6
通讯作者：
Technau, Gerhard M.
中科院分区：
文献类型：
--
作者：
Beckervordersandforth, Ruth M.;Rickert, Christof;Technau, Gerhard M.
在果蝇胚胎的中枢神经系统中，发育出几种亚型的神经胶质细胞，它们排列在特定的位置，并可能履行特定的功能。导致胶质细胞亚型的特化和分化的机制在很大程度上是未知的。通过DiI标记胶质细胞特异性Gal 4系，我们已经阐明了胚胎腹神经索中外侧胶质细胞的谱系，并将每个胶质细胞与特定的干细胞联系起来。对于纵向胶质母细胞的谱系，我们表明，它由9个细胞，获得至少四个不同的身份。大量的分子标记（其中许多代表转录因子和潜在的Gcm靶基因）表明，单个神经胶质细胞表达特定的标记组合。然而，聚类分析揭示了相似的组合代码内的细胞，和表面相关的，皮质相关的，和纵向胶质细胞的类别之间的显着差异。就基因表达而言，源自相同干细胞的神经胶质细胞可以是同质的（尽管不相同;干细胞NB 1 -1、NB 5 -6、NB 6 -4、LGB）或异质的（NB 7 -4、NB 1 -3）。除了提供一个强大的工具来分析不同遗传背景下的单个神经胶质细胞的命运，这些标记基因中的每一个都代表了参与神经胶质细胞特化或分化的候选因子。我们证明了这一点，通过分析蓖麻功能丧失突变，这会影响特定的神经胶质细胞的数量和迁移。(c)2008爱思唯尔爱尔兰有限公司保留所有权利。
In the Drosophila embryonic CNS several subtypes of glial cells develop, which arrange themselves at characteristic positions and presumably fulfil specific functions. The mechanisms leading to the specification and differentiation of glial subtypes are largely unknown. By DiI labelling in glia-specific Gal4 lines we have clarified the lineages of the lateral glia in the embryonic ventral nerve cord and linked each glial cell to a specific stem cell. For the lineage of the longitudinal glioblast we show that it consists of 9 cells, which acquire at least four different identities. A large collection of molecular markers ( many of them representing transcription factors and potential Gcm target genes) reveals that individual glial cells express specific combinations of markers. However, cluster analysis uncovers similar combinatorial codes for cells within, and significant differences between the categories of surface-associated, cortex-associated, and longitudinal glia. Glial cells derived from the same stem cell may be homogeneous ( though not identical; stem cells NB1-1, NB5-6, NB6-4, LGB) or heterogeneous (NB7-4, NB1-3) with regard to gene expression. In addition to providing a powerful tool to analyse the fate of individual glial cells in different genetic backgrounds, each of these marker genes represents a candidate factor involved in glial specification or differentiation. We demonstrate this by the analysis of a castor loss of function mutation, which affects the number and migration of specific glial cells. (c) 2008 Elsevier Ireland Ltd. All rights reserved.