Zinc oxide nanoparticles improve gut health and reduce faecal zinc excretion in piglets
Zinc oxide nanoparticles improve gut health and reduce faecal zinc excretion in piglets
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DOI：
10.1016/j.livsci.2021.104610
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发表时间：
2021-07-09
影响因子：
1.8
通讯作者：
Ma, Xianyong
中科院分区：
文献类型：
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作者：
Cui, Yiyan;Tian, Zhimei;Ma, Xianyong
本研究探讨了纳米氧化锌（ZnO-N）对仔猪生长性能、肠道健康和锌铜排泄的影响。共144头仔猪（杜洛克×长白猪×大白色，21日龄断奶;平均初始体重6.15 +/- 0.01 kg）被分配到6个组中，每个围栏6个重复，4头猪，进行26天的饲养试验。这些小组是：抗生素组（基础日粮+75 mg/kg金霉素）、氧化锌组（基础日粮+1，600 mg/kg氧化锌）和4个ZnO-N组（基础日粮+200、300、400和500 mg/kg ZnO-N）。各处理间生长性能无显著差异。ZnO-N处理提高了SOD活性（线性或二次曲线，P < 0.05），回肠铜锌超氧化物歧化酶（线性，p < 0.05），空肠和回肠中的过氧化氢酶（线性或二次曲线，P < 0.05），十二指肠和回肠的总抗氧化能力（线性，p < 0.05），和回肠中的谷胱甘肽过氧化物酶（线性，p < 0.05），和空肠中的降低的丙二醛含量（线性，p < 0.05）。随着ZnO-N含量的增加，空肠伊加、IL-6、IL-10含量呈线性下降（p < 0.05），IgM含量呈线性上升（p < 0.05）。回肠伊加和IL-10含量增加（线性和二次曲线，p < 0.05），而IgM、IgG和IL-6含量降低（线性或二次曲线，p < 0.05）。锌氮组仔猪的绒毛高度和绒毛高度与隐窝深度的比值显著高于抗生素组（p < 0.05）。日粮中添加200-500 mg/kg ZnO-N组，肝脏锌、铜和粪锌含量显著低于ZnO组（p < 0.05）。这些结果表明，日粮中的ZnO-N提高肠道抗氧化和免疫能力和肠道形态，特别是500 mg/kg的ZnO-N，也ZnO-N最小化锌含量在肝脏和粪便。
This study investigated the effects of zinc oxide nanoparticles (ZnO-N) on growth performance, gut health, and zinc and copper excretion in piglets. A total of 144 piglets (Duroc x Landrace x Large White, weaned at 21 days; mean initial weight 6.15 +/- 0.01 kg) were assigned to six groups with six replicates of four pigs per pen for a 26day feeding trial. The groups were: antibiotic group (basal diet + 75 mg/kg aureomycin), ZnO group (basal diet + 1,600 mg/kg zinc oxide), and four ZnO-N groups (basal diet + 200, 300, 400, or 500 mg/kg ZnO-N, respectively). No significant difference in growth performance was observed amongst the treatments. ZnO-N supplementation improved the activities of superoxide dismutase (linear or quadratic, p < 0.05), copper-zinc superoxide dismutase in the ileum (linear, p < 0.05), catalase in the jejunum and ileum (linear or quadratic, p < 0.05), total antioxidant capacity of duodenum and ileum (linear, p < 0.05), and glutathione peroxidase in the ileum (linear, p < 0.05), and reduced malondialdehyde content in the jejunum (linear, p < 0.05). As ZnO-N content was increased, the contents of jejunal IgA, interleukin (IL)-6, and IL-10 decreased (linear, p < 0.05), while IgM content increased (linear, p < 0.05). The contents of ileal IgA and IL-10 increased (linear and quadratic, p < 0.05), while the contents of IgM, IgG, and IL-6 decreased (linear or quadratic, p < 0.05). Villus height and the ratio of villus height to crypt depth in piglets fed with ZnO-N were higher than in the antibiotic group (p < 0.05). Pigs fed with 200-500 mg/kg ZnO-N showed lower contents of hepatic zinc and copper and faecal zinc than the ZnO group (p < 0.05). These results show that dietary ZnO-N enhanced intestinal antioxidant and immune capacity and intestinal morphology, particularly 500 mg/kg ZnO-N, and also that ZnO-N minimised zinc content in the liver and faeces.