NDRG1 contributes to retinoic acid-induced differentiation of leukemic cells
NDRG1 contributes to retinoic acid-induced differentiation of leukemic cells
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NDRG1 有助于视黄酸诱导白血病细胞分化
DOI：
10.1016/j.leukres.2008.10.016
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发表时间：
2009-08-01
影响因子：
2.7
通讯作者：
Li, Dao
中科院分区：
文献类型：
--
作者：
Chen, Su;Han, Yu-Hui;Li, Dao
N-Myc下游调控基因1 （NDRG1）蛋白在一些分化诱导剂如全反式维甲酸（ATRA）诱导的白血病细胞分化过程中被证明是上调的。然而，上调的NDRG1在该事件中的潜在作用尚不清楚。在这项工作中。我们发现，可诱导的NDRG1表达可驱动白血病U937细胞进行分化，而通过特异性小干扰RNA敲低NDRG1表达可显著拮抗atra诱导的白血病细胞分化，提示NDRG1在白血病细胞分化中的作用。此外，我们的工作表明CCAAT/增强子结合蛋白β (C/EBP β)和PU.1是重要的造血相关转录因子，可能是NDRG1在白血病细胞分化中的下游效应因子。综上所述，本研究为NDRG1蛋白在髓系白血病细胞分化中的作用提供了直接证据。(C) 2008 Elsevier Ltd版权所有。
N-Myc downstream-regulated gene 1 (NDRG1) protein has been shown to be up-regulated during leukemic cell differentiation induced by some differentiation-inducing agents such as all-trans retinoic acid (ATRA). However, the potential role of up-regulated NDRG1 in the event is greatly unknown. In this work. we show that inducible NDRG1 expression can drive leukemic U937 cells to undergo differentiation, while the knock-down of NDRG1 expression by specific small interfering RNA significantly antagonizes ATRA-induced differentiation of leukemic cells, proposing the role of NDRG1 in leukemic cell differentiation. Furthermore, our work shows that CCAAT/enhancer-binding protein beta (C/EBP beta) and PU.1, which are important hematopoiesis-related transcription factors, may act as downstream effectors of NDRG1 in leukemic cell differentiation. Taking together, this study provides direct evidence for the role of NDRG1 protein in myeloid leukemic cell differentiation. (C) 2008 Elsevier Ltd. All rights reserved.