Induction of TGF-β and IL-10 production in dendritic cells using astilbin to inhibit dextran sulfate sodium-induced colitis
Induction of TGF-β and IL-10 production in dendritic cells using astilbin to inhibit dextran sulfate sodium-induced colitis
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DOI：
10.1016/j.bbrc.2014.02.136
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发表时间：
2014-04-04
影响因子：
3.1
通讯作者：
Gong, Weijuan
中科院分区：
文献类型：
--
作者：
Ding, Yanbing;Liang, Yu;Gong, Weijuan
芦笋素是一种主要的生物活性化合物，据报道具有抗炎作用。本研究首次评价了降糖素对右旋糖酐硫酸钠（DSS）诱导的小鼠急性结肠炎的作用。腹腔注射astilbin可减轻结肠炎的严重程度，提高血清IL-10和tgf - β水平。流式细胞术检测，结肠炎小鼠脾中IL-10（+）树突状细胞（dc）和tgf - β (+) dc数量增加，CD86(+) dc、IL-12 p40(+) dc和IL-10(+) dc数量减少。给药时，也可导致结肠dc中CD103（+）的表达上调。在共培养系统中，小鼠骨髓来源的树突状细胞经天冬氨酸预处理后，CD4(+)CD25(+)Foxp3(+) T细胞的产生增强。本研究结果表明，通过介导dc的调节功能，astilbin可能成为炎症性肠病的候选药物。(C) 2014爱思唯尔公司版权所有。
Astilbin, a major bioactive compound from Rhizome smilacis glabrae, has been reported to possess anti-inflammatory properties. Our study first evaluated astilbin on dextran sulfate sodium (DSS)-induced acute colitis in mice. By intraperitoneal injection of astilbin, the severity of colitis was attenuated, and the serum levels of IL-10 and TGF-beta were increased. Using flow cytometry, a higher number of IL-10(+) dendritic cells (DCs) and TGF-beta(+) DCs and a lower number of CD86(+) DCs, IL-12 p40(+) DCs, and IL-10(+) DCs were detected in the spleen of mice with colitis after astilbin treatment. The administration of astilbin also resulted in the upregulation of CD103(+) expression in colonic DCs. In a coculture system, murine bone marrow-derived DCs pretreated with astilbin resulted in an enhanced production of CD4(+)CD25(+)Foxp3(+) T cells. The results of this study show that astilbin could be a candidate drug for inflammatory bowel disease by mediating the regulatory functions of DCs. (C) 2014 Elsevier Inc. All rights reserved.