Diversity within natural populations of eastern equine encephalomyelitis virus.
Diversity within natural populations of eastern equine encephalomyelitis virus.
复制标题
东部马脑脊髓炎病毒自然种群内的多样性。
DOI：
10.1006/viro.1993.1421
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发表时间：
1993
期刊：
影响因子：
3.7
通讯作者：
Holland,JJ
中科院分区：
文献类型：
--
作者：
Weaver,SC;Bellew,LA;Gousset,L;Repik,PM;Scott,TW;Holland,JJ
我们评估了甲病毒，东部马脑脊髓炎（EAE）病毒的自然群体内的遗传和表型多样性。RNA指纹图谱分析显示，感染宿主（未传代分离株）中的大多数群体包含一致基因型沿着少数基因型，这些基因型在1 - 3个T1抗性寡核苷酸中存在差异。突变频率似乎与其他RNA病毒的报告相似，表明HIV病毒进化的缓慢速度不受基因组复制保真度的限制。在给定的一年内，遗传多样性一般是更大的地理上遥远的分离株之间比那些从同一个传输焦点，这表明在北美的传播中的病毒是不完整的，每年。来自马里兰州和纽约的三个鸟分离物中的两个包含相对远缘的基因型，在15-19个寡核苷酸上不同。1985年从马里兰州分离出的蚊子中含有稳定的小鼠疫变种，这些变种构成了该种群的大部分。这些小斑块变异体与大斑块对应物相比，相差多达8个T1抗性寡核苷酸。在来自马里兰州和纽约的6个未传代蚊子分离株中未检测到温度敏感病毒。
We evaluated genetic and phenotypic diversity within natural populations of the alphavirus, Eastern equine encephalomyelitis (EEE) virus. RNA fingerprinting revealed that most populations within infected hosts (unpassaged isolates) contained a consensus genotype along with minority genotypes differing in one to three T1-resistant oligonucleotides. Mutation frequencies appeared to be similar to those reported for other RNA viruses, suggesting that the slow rate of EEE virus evolution is not limited by fidelity of genome replication. Within a given year, genetic diversity was generally greater among geographically distant isolates than among those from the same transmission focus, suggesting that dispersal among EEE viruses in North America is not complete annually. Two of three bird isolates from Maryland and New York contained relatively distantly related genotypes, differing in 15-19 oligonucleotides. A 1985 mosquito isolate from Maryland contained stable, small plague variants which comprised the majority of that population. These small plaque variants differed by up to eight T1-resistant oligonucleotides when compared with their large plaque counterparts. Temperature sensitive virus was not detected in six unpassaged mosquito isolates from Maryland and New York.