In vitro locomotion of allosensitized T lymphocyte clones in response to metabolites of arachidonic acid is subset specific.
In vitro locomotion of allosensitized T lymphocyte clones in response to metabolites of arachidonic acid is subset specific.
复制标题
DOI：
10.4049/jimmunol.137.2.661
复制
发表时间：
1986-07
影响因子：
4.4
通讯作者：
Mark L. Jordan;Rosemary A. Hoffman;E. Debe;Simmons Rl
中科院分区：
文献类型：
--
作者：
Mark L. Jordan;Rosemary A. Hoffman;E. Debe;Simmons Rl
本文研究了花生四烯酸代谢产物前列腺素E_2（PGE_2）和白三烯B_4（LTB_4）对克隆小鼠T淋巴细胞（来源于C57 BL/6抗DBA/2混合白细胞培养物的有限稀释分析）体外随机迁移的影响。还进行了实验以研究环加氧酶抑制剂吲哚美辛对随机淋巴细胞迁移和在PGE 2存在下淋巴细胞迁移的影响。比较克隆淋巴细胞与未致敏淋巴结淋巴细胞对PGE 2和LTB 4的反应。100 ng/ml的PGE 2显著抑制（p <0.001）T淋巴细胞辅助克隆的体外迁移，但对细胞毒性T细胞或辅助非依赖性细胞毒性（HIT）克隆细胞的随机迁移没有影响。相比之下，LTB 4在0.1和0.3 ng/ml时显著（p <0.001）增强了辅助细胞、细胞毒性细胞和“HIT”克隆细胞的随机运动。发现PGE 2和LTB 4的作用通过细胞洗涤是完全可逆的。吲哚美辛（10（-7）M）不改变任何克隆的随机迁移，特别是不影响PGE 2诱导的辅助淋巴细胞迁移的抑制。未致敏的散装淋巴结淋巴细胞迁移不受PGE 2或LTB 4。结果表明，调节淋巴细胞运动功能的环境刺激可能依赖于细胞活化，和花生四烯酸代谢产物的活化淋巴细胞的运动反应可能是亚组特异性。
The effects of the arachidonic acid metabolites prostaglandin E2 (PGE2) and leukotriene B4 (LTB4) on the in vitro random migration of cloned murine T lymphocytes (derived from limiting dilution analysis of a C57BL/6 anti-DBA/2 mixed leukocyte culture) were examined. Experiments were also performed to study the effects of the cyclooxygenase inhibitor indomethacin on both random lymphocyte migration and lymphocyte migration in the presence of PGE2. The responses of cloned lymphocytes to PGE2 and LTB4 were compared with those of unsensitized lymph node lymphocytes. PGE2 at 100 ng/ml significantly inhibited (p less than 0.001) the in vitro migration of helper clones of T lymphocytes, but had no effect on random migration of cytotoxic T cells or helper independent cytotoxic (HIT) cloned cells. In contrast, LTB4 significantly (p less than 0.001) enhanced the random locomotion of helper, cytotoxic, and "HIT" cloned cells at 0.1 and 0.3 ng/ml. The effects of both PGE2 and LTB4 were found to be completely reversible by cell washing. Indomethacin (10(-7) M) did not alter random migration of any of the clones, and in particular, did not affect the inhibition of helper lymphocyte migration induced by PGE2. Unsensitized bulk lymph node lymphocyte migration was not affected by either PGE2 or LTB4. The results suggest that modulation of lymphocyte locomotor function by environmental stimuli may depend on cellular activation, and the locomotor responses of activated lymphocytes to arachidonic acid metabolites may be subset specific.