Effects of exogenous MSH on the transformation from phaeo- to eumelanogenesis within C57BL/6J-Ay/a hairbulb melanocytes.
Effects of exogenous MSH on the transformation from phaeo- to eumelanogenesis within C57BL/6J-Ay/a hairbulb melanocytes.
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外源性 MSH 对 C57BL/6J-Ay/a 毛球黑素细胞内从褐变到真黑素生成的影响。
DOI：
10.1111/1523-1747.ep12515889
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
VanAmerongen,AW
中科院分区：
文献类型：
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作者：
Granholm,NH;VanAmerongen,AW
α促黑素细胞激素（MSH）在多大程度上是小鼠体内黑素生成的真正调节剂尚不清楚。本研究的目的是确定是否在黄色（AY/a）小鼠的毛球黑素细胞中的FSH诱导的真黑素生成模仿在遗传黑色（a/a）毛球黑素细胞中发生的真黑素生成的自然程序。我们用透射电镜定量观察了经MSH处理的Ay/a和a/a小鼠9-d再生毛中黑素体的分化。外源性α-MSH注射（5天0.15 mM MSH）的结果表明，头发的显着视觉变暗伴随着超乳至真黑素生成的不完全转化。基于几何考虑（长度、宽度、形状和面积）的3678个黑素体的发育阶段I-IV的个体发生学数据显示，MSH没有诱导从球形phaeomelanosomes到椭圆形eumelanosomes的完全转化。此外，对囊泡小叶体和基质组织的数量的观察显示，即使在5天的MSH处理后，MSH处理的AY/a黑素体保留了黑素生成的独特特征。因此，MSH诱导再生毛球的部分但不完全的真黑素生成模式。AY/a小鼠的黑素细胞。对具有“马赛克”黑素体的MSH诱导的AY/a小鼠黑素细胞中黑素合成动力学的持续研究可能有助于了解酪氨酸酶活性、基质功能、基质结构和黑素（phaeo-和/或真黑素）调节之间的基本关系）合成。
The extent to which α melanocyte-stimulating hormone (MSH) is a true in vivo regulator of melanogenesis in mice is unknown. The objective of this study was to determine if MSH-induced eurnelanogenesis in hairbulb melanocytes of yellow (AY/a) mice mimics the natural program of eumelanogenesis occurring in genetically black (a/a) hairbulb melanocytes. We conducted quantitative transmission electron microscopy on melanosome differentiation within MSH- treated regenerating 9-d hairbuibs of Ay/a and a/a mice. Results of exogenous a-MSH injections (5 dat 0.15 mM MSH) showed that the striking visual darkening of hair was accompanied by an incomplete transformation of phaco- to eumelanogenesis. Ontogenetic data on developmental stages I – IV of 3678 melanosomes based on geometric considerations (length, width, shape, and area) showed that MSH did not induce a complete transformation from spherical phaeomelanosomes to elliptical eumelanosomes. Also, observations on the number of vesiculoglobular bodies and matrix organization revealed that MSH-treated AY/a melanosomes retained distinct features of phaeomelanogenesis even after 5 d of MSH treatment. Thus, MSH induced a partial but incomplete pattern of eumelanogenesis in regenerating hairbulb. melanocytes of AY/a mice. The continued investigation of the dynamics of melanin synthesis in MSH-induced AY/a mice melanocytes possessing “mosaic” melanosomes could be productive in understanding fundamental relationships between tyrosinase activity, matrix function, matrix structure, and regulation of melanin (phaeo- and/or eumelanin) synthesis.