Anti-apoptotic effects of novel phenolic antioxidant isolated from the Pacific oyster (Crassostrea gigas) on cultured human hepatocytes under oxidative stress
Anti-apoptotic effects of novel phenolic antioxidant isolated from the Pacific oyster (Crassostrea gigas) on cultured human hepatocytes under oxidative stress
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DOI：
10.1016/j.foodchem.2014.12.066
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发表时间：
2015-06-01
期刊：
影响因子：
8.8
通讯作者：
Chiba, Hitoshi
中科院分区：
文献类型：
--
作者：
Fuda, Hirotoshi;Watanabe, Mitsugu;Chiba, Hitoshi
利用培养的人肝细胞衍生细胞（C3 A）确定了从太平洋牡蛎中分离的天然酚类抗氧化剂3，5-二羟基-4-甲氧基苄醇（DHMBA）的抗氧化和肝保护特性。DHMBA以及绿原酸（CGA）、维生素C和维生素E在62.5-500 μ M时未显示出细胞毒性。然而，丁羟甲苯，二十碳五烯酸，二十二碳六烯酸和儿茶素降低细胞活力。在促氧化剂2，2 '-偶氮二（2-脒基-丙烷）二盐酸盐（AAPH）存在下，125-500 μ M的DHMBA改善细胞活力，而CGA没有效果。DNA梯状条带形成和流式细胞术研究表明，DHMBA抑制MPH诱导的细胞凋亡和坏死。CGA无效。因此，DHMBA是一种新的，有效的抗氧化剂，有效地保护培养的肝细胞免受氧化应激引起的细胞凋亡和坏死。此外，DHMBA的浓度通过质谱测定为24.4 μ mol/kg湿牡蛎肉，和三个多酚（龙胆酸，大豆黄酮，和matairesinol）被新鉴定的牡蛎提取物。(C)2014爱思唯尔有限公司版权所有。
The antioxidant, and hepatoprotective properties of 3,5-dihydroxy-4-methoxybenzyl alcohol (DHMBA), a natural phenolic antioxidant isolated from the Pacific oyster, were defined using cultured human hepatocyte-derived cells (C3A). DHMBA showed no cytotoxicity at 62.5-500 mu M, as well as chlorogenic acid (CGA), vitamin C, and vitamin E. However, butylated hydroxytoluene, eicosapentaenoic acid, docosahexaenoic acid and catechin reduced cell viability. In the presence of the prooxidant 2,2'-azobis(2-amidino-propane) dihydrochloride (AAPH), DHMBA at 125-500 mu M improved cell viability, whereas CGA had no effect. DNA ladder formation and flow-cytometric studies indicated that DHMBA inhibited MPH-induced apoptosis and necrosis. CGA was ineffective. Thus, DHMBA is a novel, potent antioxidant, effectively protecting cultured hepatocytes from apoptosis and necrosis caused by oxidative stress. Additionally, the concentration of DHMBA was determined by mass spectrometry to be 24.4 mu mol/kg wet oyster meat, and three polyphenols (gentisic acid, daidzein, and matairesinol) were newly identified in the oyster extracts. (C) 2014 Elsevier Ltd. All rights reserved.