Detection by radioligand assay of antibodies against Borna disease virus in patients with various psychiatric disorders
Detection by radioligand assay of antibodies against Borna disease virus in patients with various psychiatric disorders
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DOI：
10.1128/cdli.12.5.671-676.2005
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发表时间：
2005-05-01
期刊：
影响因子：
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通讯作者：
Amino, N
中科院分区：
文献类型：
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作者：
Matsunaga, H;Tanaka, S;Amino, N
用保留该抗原天然形式的放射配基分析，分别在171例精神病患者的11份和19份血清中检测到了针对博尔纳病病毒核蛋白和磷蛋白的抗体。与Western blotting结果比较，分别有3份和9份血清呈一致阳性。放射配基分析显示抗体水平最高的四份血清经Western blotting均为阴性；然而，稀释和抑制试验支持阳性结果。我们的结果提示构象结构对检测人类抗博尔纳病病毒抗体的重要性。
Using a radioligand assay, which preserves the natural form of the antigen, antibodies against Borna disease virus nucleoprotein and phosphoprotein were detected in 11 and 19 sera of 171 psychiatric patients, respectively. Compared with results by Western blotting, three and nine sera were concordantly positive, respectively. The four sera showing the highest levels of antibodies by radioligand assay were all negative by Western blotting; however, dilution and inhibition tests supported the positive results. Our results suggest the importance of conformational structure to detect human anti-Borna disease virus antibodies.