THE EFFECT OF SURFACE-MEMBRANE MODIFICATIONS OF FIBROBLASTIC CELLS ON THE ENTRY PROCESS OF TRYPANOSOMA-CRUZI TRYPOMASTIGOTES
THE EFFECT OF SURFACE-MEMBRANE MODIFICATIONS OF FIBROBLASTIC CELLS ON THE ENTRY PROCESS OF TRYPANOSOMA-CRUZI TRYPOMASTIGOTES
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DOI：
10.1016/0166-6851(81)90087-6
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发表时间：
1981-01-01
影响因子：
1.5
通讯作者：
PIRAS, MM
中科院分区：
文献类型：
--
作者：
HENRIQUEZ, D;PIRAS, R;PIRAS, MM
感染T.用伴刀豆球蛋白A [Con A]植物血凝素、麦胚凝集素和蓖麻毒素I处理Vero、MA-103和鸡肌肉细胞的克氏锥鞭毛体，可减少这些细胞中的寄生虫寄生;琥珀酰化Con A也具有抑制作用。这些凝集素的作用被相应的糖半抗原所消除。胰蛋白酶和高碘酸盐处理细胞也抑制感染，以及离子载体A23187 [calcimycin]和直接破坏微管和微丝的药物，如秋水仙碱，长春碱和细胞松弛素B。成纤维细胞宿主细胞表面糖蛋白和/或质膜结构的改变可能抑制感染，表明这些细胞的表面膜在T.克鲁兹
Treatment prior to infection with T. cruzi trypomastigotes of Vero, MA-103, and chick muscle cells with concanavalin A [Con A] phytohemagglutinin, wheat germ agglutinin and ricin I results in a diminished parasite interiorization in these cells; succinylated Con A is also inhibitory. The effect of these lectins is abolished by the corresponding sugar haptens. Trypsin and periodate treatment of the cells also inhibits infection, as well as ionophore A23187 [calcimycin] and drugs that disrupt microtubules and microfilaments directly, like colchicine, vinblastine and cytochalasin B. Alteration(s) of a surface glycoprotein(s) and/or of the plasma membrane architecture of fibroblastic host cells probably inhibit infection, suggesting that the surface membrane of these cells does not play a passive role in the process of infection by T. cruzi.