Production of bioethanol using agricultural waste: banana pseudo stem
Production of bioethanol using agricultural waste: banana pseudo stem
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DOI：
10.1590/s1517-83822014000300018
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发表时间：
2014-09-01
影响因子：
2.2
通讯作者：
Gupte, Akshaya
中科院分区：
文献类型：
--
作者：
Ingale, Snehal;Joshi, Sanket J.;Gupte, Akshaya
印度是最大的香蕉（Musa acuminata）生产国之一，因此香蕉假茎是常用的农业废物，可用作木质纤维素基质。目前的研究重点是利用香蕉假茎作为生物乙醇的来源，利用不同的化学和生物预处理释放的糖来生产生物乙醇。据报道，两种真菌菌株椭圆曲霉和烟曲霉可以在甘蔗渣上产生纤维素分解酶，并在香蕉假茎上进行共培养发酵，以降解全纤维素并促进还原糖的最大限度释放。经过碱和微生物处理后获得的水解产物通过酿酒酵母NCIM 3570发酵生产乙醇。纤维素水解产物（4.1 g%）的发酵在72小时后产生最大乙醇（17.1 g/L），产率（84%）和生产率（0.024 g%/h）。本研究探讨了使用椭圆曲霉和烟曲霉对纤维素底物糖化进行真菌预处理以通过酿酒酵母 NCIM 3570 生产乙醇的一些关键方面。据观察，经过预处理的香蕉假茎可以经济地用作乙醇生产的更便宜的底物。
India is amongst the largest banana (Musa acuminata) producing countries and thus banana pseudo stem is commonly available agricultural waste to be used as lignocellulosic substrate. Present study focuses on exploitation of banana pseudo stem as a source for bioethanol production from the sugars released due to different chemical and biological pretreatments. Two fungal strains Aspergillus ellipticus and Aspergillus fumigatus reported to be producing cellulolytic enzymes on sugarcane bagasse were used under co-culture fermentation on banana pseudo stem to degrade holocellulose and facilitate maximum release of reducing sugars. The hydrolysate obtained after alkali and microbial treatments was fermented by Saccharomyces cerevisiae NCIM 3570 to produce ethanol. Fermentation of cellulosic hydrolysate (4.1 g%) gave maximum ethanol (17.1 g/L) with yield (84%) and productivity (0.024 g%/h) after 72 h. Some critical aspects of fungal pretreatment for saccharification of cellulosic substrate using A. ellipticus and A. fumigatus for ethanol production by S. cerevisiae NCIM 3570 have been explored in this study. It was observed that pretreated banana pseudo stem can be economically utilized as a cheaper substrate for ethanol production.