Accumulation, metabolites formation and elimination behavior of rac-glufosinate-ammonium and glufosinate-P in zebrafish (Danio rerio).
Accumulation, metabolites formation and elimination behavior of rac-glufosinate-ammonium and glufosinate-P in zebrafish (Danio rerio).
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外消旋草铵膦和草铵膦在斑马鱼（斑马鱼）中的积累、代谢物形成和消除行为
DOI：
10.1016/j.fochx.2022.100383
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发表时间：
2022-10-30
期刊：
影响因子：
6.1
通讯作者：
Deyu Hu
中科院分区：
文献类型：
--
作者：
Fei Wang;Qiao Lin;Xueqin Shi;Yunfang Li;Pengyu Deng;Yuping Zhang;Deyu Hu
斑马鱼样品用硅藻土、CH2Cl2和OASIS Prime HLBSPE柱净化。草甘膦在斑马鱼体内的生物积累具有对映选择性。草甘膦-P在斑马鱼体内的累积大于RAC-草甘膦。草甘膦在斑马鱼体内的消除半衰期小于2.3d，在鱼类体内产生的NAG和MPP不超过亲本培养浓度的4%。建立了高效检测斑马鱼中草甘膦对映体及其代谢物残留量的高效痕量检测方法。经二氯甲烷和OASIS Prime HLB固相萃取柱净化后，回收率为77%~104%，RSD为10.03%。斑马鱼中的定量限为0.006-0.02 mg/kg。结果表明，斑马鱼对草甘膦的吸收缓慢，在10~14天内达到稳定状态，D/L-草甘膦-铵的生物累积系数小于0.3。L-草甘膦-铵在斑马鱼体内优先积累。代谢产物N-乙酰谷氨酸(NAG)的残留量小于3-甲基膦酸(MPP)的残留量。D/L-草甘膦-铵在消除阶段的消除半衰期小于2.3天。本研究揭示了草甘膦-铵在斑马鱼水生系统中的生物积累和消除行为，为评价草甘膦-草甘膦-铵和草甘膦-P(L-草甘膦-纯铵)在鱼类体内的食品安全性提供了科学数据。
Zebrafish samples were purified with diatomaceous earth, CH2Cl2 and Oasis PRiME HLB SPE column. The bioaccumulation of glufosinate in zebrafish is enantioselective. The accumulation of glufosinate-P in zebrafish was greater than that of rac-glufosinate. The elimination half-life of glufosinate in zebrafish is less than 2.3 d. NAG and MPP produced in fish was not over 4% of the parent culture concentration. An efficient trace detection method for the determination of residues of the glufosinate enantiomers and metabolites in zebrafish by HPLC–Q-Exactive Orbitrap Mass Spectrometry was developed. After the purification of dichloromethane and Oasis PRiME HLB SPE column, the recovery ranges from 77% to 104%, with RSD < 10.03%. The limits of quantitation in zebrafish were 0.006–0.02 mg/kg. The results revealed zebrafish absorbed glufosinate slowly, reaching a steady state in 10–14 days, and the bioaccumulation factor (BCF) of D/L-glufosinate-ammonium was less than 0.3. L-glufosinate-ammonium accumulated preferentially in zebrafish. The residue of the metabolite N-acetyl glutamate (NAG) was smaller than that of 3-methyl phosphonic acid (MPP). D/L-glufosinate-ammonium had an elimination half-life of less than 2.3 days during the elimination phase. The bioaccumulation and elimination behavior of glufosinate-ammonium in zebrafish aquatic system was shown in this work, which offered scientific data for assessing the food safety of rac-glufosinate-ammonium and glufosinate-P (pure L-glufosinate-ammonium) in fish.