A rosette assay for the determination of C 1 q receptor‐bearing cells
A rosette assay for the determination of C 1 q receptor‐bearing cells
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用于测定携带 C 1 q 受体的细胞的玫瑰花结测定
DOI：
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发表时间：
1979
影响因子：
5.4
通讯作者：
A. Sobel
中科院分区：
文献类型：
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作者：
Y. Gabay;H. Perlmann;P. Perlmann;A. Sobel
描述了一种用于鉴定具有c1q受体的细胞的玫瑰花结试验。戊二醛处理的牛红细胞特异性结合c1q，制备的试剂为c1q受体介导的玫瑰结形成提供了有效的指示剂。这些受体存在于人类外周淋巴细胞亚群（主要是非T细胞）和B源性淋巴母细胞样细胞的膜上。花环的形成取决于每个指示细胞结合的c1q分子的数量，并被可溶性天然c1q和胃蛋白酶抗性c1q片段特异性抑制。这些数据，加上c1r - c1q相关的c1q结合活性降低，表明淋巴膜的c1q结合位点在胶原样部分上表达，c1q玫瑰花结的形成为人类淋巴细胞群体的分离和不表达c1q受体的吞噬细胞的分离提供了一种简单的新方法。
A rosette assay for the identification of cells with receptors for C 1q is described. Glutaraldehyde‐treated bovine erythrocytes bound C 1q specifically, and the reagent thus prepared provided a valid indicator for rosette formation mediated by C 1 q receptors. The presence of these receptors on the membrane of a subset of human peripheral lymphocytes (mainly non‐T cells) and on B‐derived lymphoblastoid cells was confirmed. Rosette formation was dependent on the number of C 1 q molecules bound per indicator cell and was specifically inhibited by soluble native C 1 q and pepsin‐resistant C 1 q fragments. These data, together with the reduced binding activity of C 1r‐C 1s‐associated C 1q, indicated that the C 1 q binding sites for lymphoid membranes are expressed on the collagen‐like moiety, C 1q rosette formation provided a simple new procedure for fractionation of human lymphocyte populations and separation from phagocytes that do not express receptors for C 1 q.