Porphyrin light-harvesting arrays constructed in the recombinant tobacco mosaic virus scaffold
Porphyrin light-harvesting arrays constructed in the recombinant tobacco mosaic virus scaffold
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DOI：
10.1002/chem.200700895
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发表时间：
2007-01-01
影响因子：
4.3
通讯作者：
Majima, Tetsuro
中科院分区：
文献类型：
--
作者：
Endo, Masayuki;Fujitsuka, Mamoru;Majima, Tetsuro
我们已经证明了在烟草花叶病毒（TMV）的超分子结构的自组装的重组TMV外壳蛋白（TMVCP）的单体，其中锌配位卟啉（ZnP）和自由基卟啉（FbP）的位置选择性地纳入多个卟啉阵列的建设。卟啉部分纳入TMV组件的物理化学性质也进行了表征。TMV-卟啉缀合物用作构建块，在适当的条件下自组装成独特的盘和杆结构，与天然TMV组装体相似。将ZnP供体和FbP受体的混合物包装在TMV组装体中，并显示出能量转移和捕光活性。通过时间分辨荧光光谱研究了TMV组装体中卟啉阵列的详细光物理性质，并确定能量转移速率为3.1-6.4 × 10（9）s（-1）。结果表明，卟啉被放置在TMV组装的预期位置。
We have demonstrated the construction of multiple porphyrin arrays in the tobacco mosaic virus (TMV) supramolecular structures by self-assembly of recombinant TMV coat protein (TMVCP) monomers, in which Zn-coordinated porphyrin (ZnP) and free-base porphyrin (FbP) were site-selectively incorporated. The photophysical properties of porphyrin moieties incorporated in the TMV assemblies were also characterized. TMV-porphyrin conjugates employed as building blocks self-assembled into unique disk and rod structures under the proper conditions as similar to native TMV assemblies. The mixture of a ZnP donor and an FbP acceptor was packed in the TMV assembly and showed energy transfer and light-harvesting activity. The detailed photo-physical properties of the arrayed porphyrins in the TMV assemblies were examined by time-resolved fluorescence spectroscopy, and the energy transfer rates were determined to be 3.1-6.4 x 10(9) s(-1). The results indicate that the porphyrins are placed at the expected positions in the TMV assemblies.