Aluminum mediates compositional alterations of polar lipid classes in maize seedlings
Aluminum mediates compositional alterations of polar lipid classes in maize seedlings
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DOI：
10.1016/j.phytochem.2005.06.028
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发表时间：
2005-08-01
期刊：
影响因子：
3.8
通讯作者：
El Ferjani, E
中科院分区：
文献类型：
--
作者：
Chaffai, R;Marzouk, B;El Ferjani, E
研究了铝胁迫下玉米根系和芽部脂质组成的变化。100亩ma1处理4d后，根系生长受到抑制，而茎部生长不受影响。在根中，总脂肪酸双键指数（DBI）的降低可能表明膜流动性的降低。总脂质（TL）下降49%，磷脂（PL）、磷脂酰胆碱（PC）和磷脂酰肌醇（PI）增加约3倍。MGDG增加到2倍，但DGDG没有明显变化。甾醇脂（SL）增加69%。铝胁迫植物根系的SL/PL比值从2.64下降到1.52，MGDG/DGDG比值从0.45上升到1.06。通过丙二醛（MDA）浓度的增加可以看出，A1导致了处理过的植物根部的氧化应激。在芽部，脂肪酸组成的变化与除DGDG降低外的所有脂类DBI增加有关。PG为脂类，其脂肪酸组成变化较大。在处理过a1的植株中，TL、PL、SL和MDA的浓度均未发生显著变化。PE水平变化不明显，PI、PG升高，PC降低。然而，A1导致GL水平下降87%。MGDG和DGDG分别下降到19倍和8倍。A1对极性脂质的有害作用可能是通过直接干预质膜和/或改变细胞代谢引起的。(c) 2005 Elsevier Ltd版权所有。
Changes in lipid composition were investigated on maize roots and shoots under aluminum stress. After 4d exposure to 100 mu M A1, root growth was inhibited while shoot growth was not affected. In roots, the decrease of the DBI (double bond index) of total fatty acids may signal a decrease in membrane fluidity. The total lipids (TL) decreased by 49%, but phospholipids (PL), phosphatidylcholine (PC) and phosphatidylinositol (PI) increased to approximately 3-fold. The MGDG increased to 2-fold but no significant change was found in the DGDG. The steryl lipids (SL) increased by 69%. The SL/PL ratio decreased from 2.64 to 1.52 and the MGDG/DGDG ratio increased from 0.45 to 1.06 in roots of Al-stressed plants. A1 leads to oxidative stress in roots of treated plants as indicated by the increase of malondialdehyde (MDA) concentrations. In shoots, changes in fatty acid composition were associated with an increase of the DBI in all lipid classes except that of the DGDG decreased. The PG was the lipid class which shows the large variation of fatty acid composition. No significant changes were found either for TL, PL, SL or MDA concentrations in shoots of A1-treated plants. While PE levels did not show significant change, PI and PG increased and PC decreased. However, the A1 caused 87% decrease in the GL levels. The MGDG and DGDG decreased to 19 and 8-fold, respectively. The deleterious effects of A1 on polar lipids could be caused by a direct intervention of A1 on plasma membrane and/or alteration of cell metabolism. (c) 2005 Elsevier Ltd. All rights reserved.