The effect of fasting on the concentrations of total proteins and of uric acid as well as on aminotransferase activity in duckling blood plasma
The effect of fasting on the concentrations of total proteins and of uric acid as well as on aminotransferase activity in duckling blood plasma
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禁食对雏鸭血浆总蛋白、尿酸浓度及转氨酶活性的影响
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发表时间：
2008
期刊：
影响因子：
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通讯作者：
J. Piršljin
中科院分区：
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作者：
Ivana Tlak;S. Milinković;Z. Stojević;J. Piršljin
本文研究了禁食6天对北京鸭雏鸭血清总蛋白、尿酸浓度以及天冬氨酸氨基转移酶[天冬氨酸：2-酮戊二酸氨基转移酶（AST）; EC 2.6.1.1]和丙氨酸氨基转移酶[L-丙氨酸：2-酮戊二酸氨基转移酶（ALT）; EC 2.6.1.2]活性的影响。在28日龄时，将雏鸭分为两组：正常喂养的对照组（n = 28）和禁食6天的组。试验组8只，对照组7只，分别于禁食第3、4、5、6天断头处死。在实验组和对照组中同时从颈部采集用于分析的血液，肝素用作抗凝剂。在所获得的血浆中，通过荧光定量法评估总蛋白和尿酸的浓度以及AST和ALT活性水平。在整个试验期间，血浆中总蛋白的浓度禁食雏鸭显着低于对照值：在第3天，P = 0.0239，并在第4，5和6天，P = 0.0012。试验5 d后，试验组雏鸭的尿酸浓度显著高于对照组（P = 0.018），而对照组雏鸭的尿酸浓度在整个试验期内最低。与对照组相比，空腹第5天后AST活性显著降低（P = 0.0276），空腹第4天后ALT活性显著降低（P = 0.0410）。整个试验期间总蛋白浓度低以及上述酶活性降低可能是由于禁食期间胃肠道氨基酸缺乏导致体内蛋白质再生减少的结果。
The effect of a six-day fasting period on the variations of total protein and uric acid concentrations as well as on the activities of aspartate aminotransferase [aspartate:2-oxoglutarate aminotransferase (AST) ; EC 2.6.1.1] and alanine aminotransferase [L-alanine:2-oxoglutarate aminotransferase (ALT) ; EC 2.6.1.2] was investigated in Pekin Duck ducklings. At the age of 28 days, ducklings were divided into two groups: a normally fed control group (n = 28), and a group that was submitted to a six-day long fasting. Eight ducklings from the experimental group and seven ducklings belonging to the control group were sacrifised by decapitation after the 3rd, the 4th, the 5th, and the 6th day of fasting. The blood for analysis was sampled from the neck, simultaneously in the experimental and in the control group, heparin being used as the anticoagulant. In the obtained blood plasma, the concentrations of total proteins and of uric acid as well as the levels of AST and ALT activities were assessed spectrophotometrically. Over the entire trial period, the concentration of total proteins in the blood plasma of fasting ducklings was significantly lower than the control values: on day 3, P = 0.0239, and on days 4, 5, and 6, P = 0.0012. After the period of five days of the trial, a significantly higher (P = 0.018) value of uric acid concentration was measured in experimental ducklings than in controls, but at the same time, the lowest uric acid concentration in the entire trial period was found in the control group. In relation to the control group, a significantly lower AST activity was found after the 5th day of fasting (P = 0.0276), and a lower ALT activity after the 4th day of fasting (P = 0.0410). Low concentrations of total proteins over the entire trial period as well as reduced activities of the afore mentioned enzymes were probably a consequence of a reduced protein regeneration in the body due to the deficiency of amino acids from the gastro-intestinal tract during fasting.