Superparamagnetic iron oxide nanoparticles modified with dimyristoylphosphatidylcholine and their distribution in the brain after injection in the rat substantia nigra
Superparamagnetic iron oxide nanoparticles modified with dimyristoylphosphatidylcholine and their distribution in the brain after injection in the rat substantia nigra
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二肉豆蔻酰磷脂酰胆碱修饰的超顺磁性氧化铁纳米颗粒及其注射到大鼠黑质后在脑中的分布
DOI：
10.1016/j.msec.2017.08.049
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发表时间：
2017
影响因子：
7.9
通讯作者：
Tan Jie
中科院分区：
文献类型：
--
作者：
Su Lichao;Zhang Baolin;Huang Yinping;Zhang Hao;Xu Qin;Tan Jie
纳米粒子在脑中的亚细胞分布对其生物学应用具有重要意义。我们合成并表征了聚乙二醇（PEG）和聚乙烯亚胺（PEI）修饰的超顺磁性氧化铁纳米粒子（PEG/PEI-SPIONs）和二肉豆蔻酰磷脂酰胆碱（DMPC）修饰的超顺磁性氧化铁纳米粒子（DMPC-SPIONs）。将纳米颗粒单侧注射到大鼠大脑的左侧黑质中。在注射后24 h通过ICP-OES（电感耦合等离子体发射光谱仪）和TEM（透射电子显微镜）检查纳米颗粒在大鼠左脑中的分布。注射DMPC-SPION和PEG/PEI-SPION后24 h，在嗅球、颞叶、额叶皮质、丘脑和脑干中发现铁。在大鼠黑质，DMPC-SPION主要分布于轴突周围的髓鞘内、髓鞘上或细胞膜上，也有部分分布于细胞内。作为比较，在注射PEG/PEI-SPION后24小时，在大鼠脑中发现较少的铁。我们的实验表明，DMPC修饰SPION是一种安全有效的增加SPION在细胞膜上分布的方法。这项工作是令人鼓舞的进一步研究使用DMPC-SPION的有效药物输送或深部脑刺激的神经元在磁场中。
The subcellular distributions of nanoparticles in the brain are important for their biological application. We synthesized and characterized the superparamagnetic iron oxide nanoparticles (SPIONs) modified with poly (ethylene glycol) (PEG) and polyethylenimine (PEI) (PEG/PEI-SPIONs), and with dimyristoylphosphatidylcholine (DMPC) (DMPC-SPIONs). The nanoparticles were unilaterally injected into the left substantia nigra of rat brains. The distributions of the nanoparticles in the left brains of the rats were examined by ICP-OES (inductively coupled plasma optical emission spectrometer) and TEM (transmission electron microscopy) at 24 h after the injection. Iron was found in the olfactory bulb, temporal lobe, frontal cortex, thalamus and brain stem at 24 h after the injection of DMPC-SPIONs and PEG/PEI-SPIONs. In the rat substantia nigra, most DMPC-SPIONs were distributed in and on the myelin sheath around axons or on cell membranes, some were in cells. As a comparison, less iron was found in the rat brains at 24 h after the injection of PEG/PEI-SPIONs. Our experiments suggest DMPC modification on SPIONs be a safe and effective method for increasing SPIONs distribution on the cell membranes. This work is encouraging for further study on using DMPC-SPIONs for efficient drug delivery or for deep brain stimulation of neurons in a magnetic field.