Microleakage of new dentin bonding systems using human and bovine teeth.
Microleakage of new dentin bonding systems using human and bovine teeth.
复制标题
使用人和牛牙齿的新型牙本质粘合系统的微渗漏。
作者：
Reeves Gw;Fitchie Jg;Hembree Jh;Aaron D. Puckett
本研究的目的是双重的：评价三种牙本质粘接系统的微渗漏行为，并确定在研究各种材料的微渗漏时，牛牙是否与人牙具有可比性。评价的材料为Scotchbond多功能粘合剂、Prisma Universal Bond 3和All-Bond 2。所有三种粘接系统与Prisma APH混合复合材料结合使用，以比较微渗漏行为。在牙骨质-釉质连接处切割60个5级制备物，用于包含30个人牙和30个牛牙的组。在釉质边缘放置1 mm 45度斜面。根据使用的牙本质粘结系统对牙齿进行分组，然后根据制造商的说明进行修复。修复后，将每组牙齿保存在37 ℃的蒸馏水中3天。然后将牙齿在4摄氏度和58摄氏度之间热循环100次，并在37摄氏度的蒸馏水中再循环4天。然后用指甲油将牙齿密封至距离修复体边缘1 mm处，并置于45 Ca同位素中2小时。然后将牙齿切片并放置在X射线胶片上以产生放射自显影。使用以下量表分别评价釉质和牙本质边缘的微渗漏：0 =无渗漏，1 =同位素渗透到轴壁距离的1/2以下，2 =同位素渗透到轴壁距离的1/2以上但未达到轴壁，3 =同位素渗透到轴壁或更远。使用Mann-Whitney U和Kruskal-Wallis检验对材料进行相互比较。将所有材料的龈缘与切缘进行比较。人类基质的切缘和牙龈位置之间的微渗漏无统计学显著差异，但牛基质的牙龈微渗漏有统计学显著性差异。对于切缘处的人体基质，All-Bond 2的泄漏量明显大于Scotchbond Multi-Purpose。对于牛基质，All-Bond 2在牙本质和牙釉质边缘的微渗漏显著高于Prisma Universal Bond 3。人体基质的粘合系统之间的微渗漏没有统计学显着差异。结果表明，人牙和牛牙基质的微渗漏行为无显著差异，这一结果支持牛牙用于体外微渗漏研究。
The objective of this study was twofold: to evaluate the microleakage behavior of three dentin bonding systems and to determine if bovine teeth are comparable substrates to human teeth when studying the microleakage of various materials. The materials evaluated were Scotchbond Multi-Purpose Adhesive, Prisma Universal Bond 3, and All-Bond 2. All three bonding systems were used in combination with Prisma APH hybrid composite for comparison of microleakage behavior. Sixty class 5 preparations were cut at the cementoenamel junction for groups containing 30 human and 30 bovine teeth. A 1 mm 45 degree bevel was placed at the enamel margin. Teeth were grouped according to the dentin bonding system used and then restored according to the manufacturer's directions. After restoration, the teeth from each group were stored in distilled water at 37 degrees C for 3 days. The teeth were then thermocycled between 4 degrees C and 58 degrees C for 100 cycles and returned to distilled water at 37 degrees C for an additional 4 days. The teeth were then sealed with nail polish up to 1 mm from the margins of the restoration and placed in 45Ca isotope for 2 hours. The teeth were then sectioned and placed on x-ray film to produce autoradiographs. Microleakage was evaluated for the enamel and dentin margins separately using the following scale: 0 = no leakage, 1 = penetration of isotope to less than 1/2 the distance to the axial wall, 2 = penetration of isotope greater than 1/2 of the distance to the axial wall but short of the axial wall, and 3 = penetration of isotope to the axial wall or beyond. The materials were compared to each other using the Mann-Whitney U and Kruskal-Wallis tests. The gingival margins were compared to the incisal margins for all materials. No statistically significant differences in microleakage were revealed between the incisal and gingival location for human substrates, but there was statistically significant greater gingival microleakage for bovine substrates. All-Bond 2 leaked significantly more than Scotchbond Multi-Purpose for human substrates at the incisal margin. All-Bond 2 had significantly more microleakage than Prisma Universal Bond 3 at both dentin and enamel margins for the bovine substrate. There were no statistically significant differences in microleakage among the bonding systems for the human substrate. No statistically significant differences between the microleakage behavior of human and bovine substrates were found. These results support the use of bovine teeth for in vitro microleakage studies.