The Arabidopsis ABCG13 transporter is required for flower cuticle secretion and patterning of the petal epidermis
The Arabidopsis ABCG13 transporter is required for flower cuticle secretion and patterning of the petal epidermis
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DOI：
10.1111/j.1469-8137.2010.03608.x
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发表时间：
2011-01-01
期刊：
影响因子：
9.4
通讯作者：
Aharoni, Asaph
中科院分区：
文献类型：
--
作者：
Panikashvili, David;Shi, Jian Xin;Aharoni, Asaph
前期研究表明，ABCG 11和ABCG 12是两种ATP结合盒（ABC）转运蛋白，参与表皮脂质的胞外分泌。在这里，我们的特点ABCG13，第三支成员，扩大我们有限的知识，关于组装的植物角质层。我们分离出一个abcg13敲除突变体，并使用RNAi和人工microRNA的方法来研究ABCG13功能丧失的影响。这些植物随后被用来进行角质层脂质组成和细胞学观察的详细分析。ABCG13功能丧失导致角质层相关的表型，仅限于花，包括器官间后生殖融合。除了花角质单体的显着减少，宏观形态和abcg13花瓣表皮的微形态受到强烈影响。我们还发现ABCG 13在花中高度表达，主要在花瓣和心皮中，表明ABCG 13是花表皮脂质运输所必需的。这项工作介绍了一个新的组成部分，最近出现的遗传网络，使原型外部的拟南芥花。虽然关于ABCG12进化枝成员的底物特异性的问题仍然是开放的，这些发现将有助于未来的调查关于可能参与角质层组装的半尺寸ABCG型转运蛋白之间的相互作用。
P>Previous studies showed that ABCG11 and ABCG12, two ATP-binding-cassette (ABC) transporters, are required for cuticular lipids extracellular secretion. Here, we characterized ABCG13, a third clade member, to widen our limited knowledge regarding assembly of the plant's cuticle.We isolated an abcg13 knockout mutant and used RNAi and artificial microRNA approaches to study the effect of ABCG13 loss-of-function. These plants were subsequently used to conduct a detailed analysis of cuticular lipids composition and cytological observations.ABCG13 loss-of-function resulted in cuticle-related phenotypes that were restricted to flowers, including inter-organ post-genital fusions. Apart from a significant reduction in flower cutin monomers, the macromorphology and micromorphology of abcg13 petal epidermis was strongly affected. We also found that ABCG13 is highly expressed in flowers, predominantly in petals and carpels.The results suggest that ABCG13 is required for the transport of flower cuticular lipids. This work introduces a new component to the recently emerging genetic network that makes the archetypal exterior of Arabidopsis flowers. While the question regarding the substrate specificity of the ABCG12-clade members remains open, these findings will facilitate future investigations regarding the interaction between the half-size ABCG-type transporters that likely take part in cuticle assembly.