SURFACTANT PROTEIN-C IS EXPRESSED IN ALVEOLAR TYPE-II CELLS BUT NOT IN CLARA CELLS OF RAT LUNG
SURFACTANT PROTEIN-C IS EXPRESSED IN ALVEOLAR TYPE-II CELLS BUT NOT IN CLARA CELLS OF RAT LUNG
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DOI：
10.1165/ajrcmb/6.6.594
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发表时间：
1992-06-01
影响因子：
6.4
通讯作者：
SHANNON, JM
中科院分区：
文献类型：
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作者：
KALINA, M;MASON, RJ;SHANNON, JM
表面活性剂相关蛋白在细支气管细胞中的表达仍有待确定。我们使用原位杂交技术来鉴定成年和胎鼠肺中表面活性物质相关蛋白A、B和C（SP-A、SP-B和SP-C）的信息表达位点。这些信息的表达原位杂交也比较了SP-A的免疫细胞化学定位。SP-A的定位被用来确定II型细胞和无纤毛细支气管上皮细胞（克拉拉）在这些部分。SP-A、SP-B和SP-C的cRNA反义探针似乎在II型细胞上杂交。正义探针显示没有定位或明显的特异性杂交。SP-A和SP-B的信息也在无纤毛细支气管上皮（Clara）细胞中发现。然而，在这些细胞中没有观察到SP-C的信息。从终末到大细支气管的Clara细胞缺乏可检测到的SP-C mRNA。在I型细胞、肺泡巨噬细胞、间质细胞或血管细胞中未检测到表面活性蛋白mRNA的表达。同样，在胎鼠肺中，细支气管细胞中检测到SP-A和SP-B的信息，但未检测到SP-C的信息。我们的结论是，大鼠Clara细胞不表达SP-C mRNA，因此SP-C可以被视为大鼠II型细胞的特异性标志物。
The expression of the surfactant-associated proteins in bronchiolar cells remains to be defined. We used in situ hybridization to identify sites of message expression of the surfactant-associated proteins A, B, and C (SP-A, SP-B, and SP-C) in adult and fetal rat lung. The expression of these messages by in situ hybridization was also compared with the localization of SP-A by immunocytochemistry. The localization of SP-A was used to identify type II cells and nonciliated bronchiolar epithelial (Clara) cells in these sections. The cRNA antisense probes for SP-A, SP-B, and SP-C appeared to hybridize over type II cells. Sense probes showed no localization or apparent specific hybridization. Messages for both SP-A and SP-B were also found in nonciliated bronchiolar epithelial (Clara) cells. However, no message for SP-C was observed in these cells. Clara cells from terminal to large bronchioles lacked detectable mRNA for SP-C. Expression of surfactant protein mRNAs was not detectable in type I cells, alveolar macrophages, interstitial cells, or vascular cells. Similarly, in fetal rat lung the messages for SP-A and SP-B but not SP-C were detected in bronchiolar cells. We conclude that rat Clara cells do not express SP-C mRNA, and thus SP-C can be regarded as a specific marker for rat type II cells.