EXPRESSION OF MYELOID DIFFERENTIATION ANTIGENS ON NORMAL AND MALIGNANT MYELOID CELLS
EXPRESSION OF MYELOID DIFFERENTIATION ANTIGENS ON NORMAL AND MALIGNANT MYELOID CELLS
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DOI：
10.1172/jci110348
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发表时间：
1981-01-01
影响因子：
15.9
通讯作者：
SCHLOSSMAN, SF
中科院分区：
文献类型：
--
作者：
GRIFFIN, JD;RITZ, J;SCHLOSSMAN, SF
一系列单克隆抗体的特征在于，其定义了人髓样细胞的4种表面抗原（MY 3、MY 4、MY 7和MY 8）。它们源自（小鼠）NS-I浆细胞瘤细胞系与用人急性粒单核细胞白血病细胞免疫的小鼠的脾细胞的融合。MY 3和MY 4由正常单核细胞和> 90%的急性单核细胞白血病或急性髓单核细胞白血病患者表达，但在其他类型的髓性白血病中检测到的频率要低得多。MY 7由粒细胞、单核细胞和5%的正常骨髓细胞表达。在所有检测的急性髓细胞白血病（AML）患者（72例患者）中，79%表达MY 7，而没有任何AML亚型的优先表达。MY 8由正常单核细胞、粒细胞、所有过氧化物酶阳性骨髓细胞和50%的AML患者表达。MY 3、MY 4和MY 8定义了髓样分化抗原，因为它们在髓样前体细胞上检测不到，并且出现在分化的离散阶段。这些抗原不被淋巴细胞、红细胞、血小板或淋巴恶性肿瘤表达。定义这些抗原的单克隆抗血清已被用于研究正常骨髓细胞和恶性骨髓细胞系的分化。
A series of monoclonal antibodies were characterized that define 4 surface antigens (MY3, MY4, MY7 and MY8) of human myeloid cells. They were derived from a fusion of the (mouse) NS-I plasmacytoma cell line with splenocytes from a mouse immunized with human acute myelomonocytic leukemia cells. MY3 and MY4 are expressed by normal monocytes and by > 90% of patients with acute monocytic leukemia or acute myelomonocytic leukemia but are detected much less often on other types of myeloid leukemia. MY7 is expressed by granulocytes, monocytes and 5% of normal bone marrow cells. Of all acute myeloblastic leukemia (AML) patients tested (72 patients), 79% expressed MY7 without preferential expression by any AML subtype. MY8 is expressed by normal monocytes, granulocytes, all peroxidase-positive bone marrow cells and 50% of AML patients. MY3, MY4 and MY8 define myeloid differentiation antigens in that they are not detected on myeloid precursor cells and appear at discrete stages of differentiation. These antigens are not expressed by lymphocytes, erythrocytes, platelets or lymphoid malignancies. The monoclonal antisera defining these antigens have been used to study differentiation of normal myeloid cells and malignant myeloid cell lines.