Molecular Detection and Assessment of Risk Factors for Tick-Borne Diseases in Sheep and Goats from Turkey
Molecular Detection and Assessment of Risk Factors for Tick-Borne Diseases in Sheep and Goats from Turkey
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DOI：
10.2478/s11686-020-00207-0
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发表时间：
2020-05-06
影响因子：
1.5
通讯作者：
Xuan, Xuenan
中科院分区：
文献类型：
--
作者：
Benedicto, Byamukama;Ceylan, Onur;Xuan, Xuenan
蜱传疾病主要是泰勒虫病、巴贝斯虫病和无形体病，在包括土耳其在内的全球牲畜中造成重大经济损失。土耳其小反刍动物的蜱传病原体已被广泛研究，但有关分子特征和疾病发生的信息仍然有限。方法采用显微镜和分子生物学方法对泰勒虫进行鉴定。羊巴氏无形体、羊无形体和嗜吞噬细胞无形体。从土耳其收集了133份蜱虫感染的小反刍动物（105只绵羊和28只山羊）的血液样本：一半的动物有蜱传疾病感染的临床体征。结果PCR法和镜检法分别有90.2%和45.1%的标本至少检出一种病原体。总的来说，A.嗜吞噬细胞菌B. B. ovis、黑腹拟步行虫A.羊泰勒虫（Theileria spp.）分别为66.7%、62.4%、46.6%和7.0%。133份样本中有59份（44.4%）同时感染两种或两种以上病原体。性，季节和B。ovis阳性是临床疾病发生的重要危险因素。基于B的序列和系统发育分析。ovis 18S small subunit rRNA，A.绵羊主要表面蛋白4、泰勒虫属（Theileria spp.）18SrRNA和A.嗜吞噬细胞菌16S rRNA基因表明，在这项研究中的菌株聚集在一起，与以前收集的土耳其和其他国家的支持分支。结论本研究显示B. ovis是与来自土耳其的小反刍动物的临床和致死性病例相关的最重要的病原体。女性和夏季与疾病的风险增加有关。本研究表明，包括A.具有兽医和公共卫生重要性。
Background Tick-borne diseases mainly, theileriosis, babesiosis and anaplasmosis cause significant economic losses in livestock globally, including Turkey. The tick-borne pathogens of small ruminants in Turkey have been studied widely but information on molecular characterization and disease occurrence is still limited. Methods In this study, both microscopy and molecular detection and characterization for Theileria spp. Babesia ovis, Anaplasma ovis and Anaplasma phagocytophilum was conducted. A total of 133 blood samples of tick-infested small ruminants (105 sheep and 28 goats) were collected from Turkey: half of the animals had clinical signs of tick-borne disease infections. Results Using PCR assays and microscopy, 90.2% and 45.1% of the samples were positive for at least one pathogen, respectively. Overall, the infection rates of A. phagocytophilum, B. ovis, A. ovis, Theileria spp. were 66.7%, 62.4%, 46.6% and 7.0%, respectively. Fifty-nine of the 133 (44.4%) samples were co-infected with two or more pathogens. Sex, season and B. ovis positivity were significant risk factors for occurrence of clinical disease. Sequence and phylogenetic analysis based on B. ovis 18S small subunit rRNA, A. ovis major surface protein 4, Theileria spp. 18S rRNA and A. phagocytophilum 16S rRNA genes showed that the isolates in this study clustered together in well-supported clades with those previously collected from Turkey and other countries. Conclusions The study shows B. ovis as the most significant pathogen associated with clinical and fatal cases in small ruminants from Turkey. Female sex and summer season are associated with increased risk of the disease. This study shows high infection rates with the pathogens among small ruminants including A. phagocytophilum which has veterinary and public health importance.