Basic fibroblast growth factor (bFGF) acts intracellularly to cause the transdifferentiation of avian neural crest-derived Schwann cell precursors into melanocytes.
Basic fibroblast growth factor (bFGF) acts intracellularly to cause the transdifferentiation of avian neural crest-derived Schwann cell precursors into melanocytes.
复制标题
DOI：
--
复制
发表时间：
1993-08
期刊：
影响因子：
4.6
通讯作者：
Larry S. Sherman;K. Stocker;Richard S. Morrison;G. Ciment
中科院分区：
文献类型：
--
作者：
Larry S. Sherman;K. Stocker;Richard S. Morrison;G. Ciment
我们之前发现，培养的来自胚胎鹌鹑周围神经的神经嵴衍生细胞（主要由雪旺细胞前体组成）在用碱性成纤维细胞生长因子（bFGF）或12-O-十四烷酰佛波醇-13-乙酸酯（TPA）处理后产生黑色素细胞。在这里，我们发现针对 bFGF mRNA 转录物的两个区域的反义脱氧寡核苷酸阻断了 TPA 诱导的雪旺细胞前体的转分化。有义或乱序反义对照寡核苷酸在这方面均没有任何作用。 TPA 增加了细胞裂解物中的 bFGF 蛋白表达，但在这些培养物的条件培养基中没有增加，并且这种表达局限于细胞核和细胞质。此外，bFGF 中和抗体和肌醇六磷酸 (InsP6) 均能抑制外源性 bFGF 引起的色素沉着，但对 TPA 诱导的黑色素生成没有影响，表明这些细胞不释放 bFGF。这些数据表明 bFGF 对于 TPA 诱导的雪旺细胞前体转分化为黑素细胞是必需的，并且 bFGF 通过内分泌机制发挥作用。
We previously found that cultured neural crest-derived cells from embryonic quail peripheral nerves, which consist mostly of Schwann cell precursors, gave rise to melanocytes following treatment with basic fibroblast growth factor (bFGF) or 12-O-tetradecanoyl phorbol-13-acetate (TPA). Here, we show that antisense deoxyoligonucleotides targeted against two regions of the bFGF mRNA transcript blocked this TPA-induced transdifferentiation of Schwann cell precursors. Neither sense nor scrambled antisense control oligonucleotides had any effect in this regard. TPA increased bFGF protein expression in cell lysates but not in conditioned media from these cultures, and this expression was localized to the nucleus and cytoplasm. Furthermore, bFGF-neutralizing antibodies and inositol-hexakisphosphate (InsP6) both inhibited pigmentation caused by exogenous bFGF, but had no affect on TPA-induced melanogenesis, suggesting that bFGF is not released by these cells. These data indicate that bFGF is necessary for the TPA-induced transdifferentiation of Schwann cell precursors into melanocytes and that bFGF acts via an intracrine mechanism.