The intrinsic fluorescence of the recombinant human leukocyte interferon-αA and fibroblast interferonβ1
The intrinsic fluorescence of the recombinant human leukocyte interferon-αA and fibroblast interferonβ1
复制标题
重组人白细胞干扰素-αA和成纤维细胞干扰素β1的内源荧光
DOI：
10.1016/0006-291x(90)91465-5
复制
发表时间：
1990
期刊：
影响因子：
--
通讯作者：
A. Strongin
中科院分区：
文献类型：
--
作者：
S. I. Borukhov;A. Strongin
The environment of Trp residues of the recombinant human interferons has been studied by the analysis of the emission spectra of native and denatured proteins at pH 1,5–8,5 and temperature 10–65°C in the presence and absence of the anionic, cationic and neutral to charge contact quenchers-KI, CsCl and acrylamide, respectively. The obtained data allow to suppose that in IFN-αA and IFN-β1 Trp141interacts with Arg145and one or several from the following residues-Phe124, Ile127, Tyr130, Leu131, whereas Trp77- with Arg33and Phe36, Phe78, Leu81or Leu82(Ile81or Val82for IFN-β1).
