In ovo eye electroporation.
In ovo eye electroporation.
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DOI：
10.1007/978-1-61779-848-1_5
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发表时间：
2012-01-01
期刊：
影响因子：
--
通讯作者：
Tiwari, Sarika
中科院分区：
文献类型：
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作者：
Belecky-Adams, Teri L;Hudson, Scott R;Tiwari, Sarika
电穿孔技术成功地将DNA等大分子引入鸡胚胎已有至少15年的历史。将纯化的质粒DNA显微注射到胚胎中，然后在胚胎上施加一系列低压电脉冲，使裸露的DNA进入细胞。在进入细胞质后，DNA被运输到细胞核，在那里它被瞬时表达。这项强大的技术对于涉及在不同发育时间点的感兴趣基因的过度表达、错误表达和敲除的研究是有用的。
Electroporation has been used successfully to introduce macromolecules such as DNA into the chick embryo for at least 15 years. Purified plasmid DNA is microinjected into embryo and then a series of low voltage electrical pulses are applied to the embryo which allows naked DNA to enter cells. Following entrance into the cytoplasm, the DNA is transported to the nucleus where it is transiently expressed. This powerful technique is useful for studies involving overexpression, misexpression, and knockdown of genes of interest at a variety of developmental timepoints.