CD147/basigin reflects renal dysfunction in patients with acute kidney injury
CD147/basigin reflects renal dysfunction in patients with acute kidney injury
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DOI：
10.1007/s10157-013-0916-3
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发表时间：
2014-10-01
影响因子：
2.3
通讯作者：
Maruyama, Shoichi
中科院分区：
文献类型：
--
作者：
Nagaya, Hiroshi;Kosugi, Tomoki;Maruyama, Shoichi
急性肾小管坏死（ATN）描述了一种固有的急性肾损伤（阿基）形式，其由持续的低灌注和随后的免疫系统激活引起。糖基化跨膜蛋白CD 147/basigin参与肾缺血和纤维化的发病机制。本研究收集2008 - 2012年间行肾活检的24例AKI患者（对照组12例，ATN组12例）的血浆和尿标本，探讨CD 147是否能反映AKI患者的病理特征和肾功能障碍。在另一项研究中，2004年入组了接受开放手术治疗腹主动脉瘤（AAA）的患者。我们分别收集了7例阿基患者和33例非阿基患者的尿液和血浆样本。在这些实验中，血浆和尿液中的CD 147，和尿中的l-脂肪酸结合蛋白（l-FABP）的水平进行了测定，并通过免疫组化检查前者在肾脏中的表达。在活检组织中的ATN与严重的组织学特征，CD 147诱导显着存在于炎症细胞，如巨噬细胞和淋巴细胞在受损的肾小管，但不是在受损的肾小管萎缩。ATN患者的血浆和尿CD 147水平均显著升高;与尿L-FABP相比，这两个值与肾功能不全的相关性更大。在接受开放性AAA手术的患者中，阿基患者的尿液和血浆CD 147值在术后第1天显著高于非AKI患者，与尿液l-FABP的特征相似。CD 147在检测阿基的能力方面突出，并且可能允许开始预先用药。
Acute tubular necrosis (ATN) describes a form of intrinsic acute kidney injury (AKI) that results from persistent hypoperfusion and subsequent activation of the immune system. A glycosylated transmembrane protein, CD147/basigin, is involved in the pathogenesis of renal ischemia and fibrosis. The present study investigated whether CD147 can reflect pathological features and renal dysfunction in patients with AKI.Plasma and spot urine samples were collected from 24 patients (12 controls and 12 with ATN) who underwent renal biopsy between 2008 and 2012. In another study, patients undergoing open surgery to treat abdominal aortic aneurysms (AAAs) were enrolled in 2004. We collected urine and plasma samples from seven patients with AKI and 33 patients without AKI, respectively. In these experiments, plasma and urinary CD147, and urinary l-fatty acid-binding protein (l-FABP) levels were measured, and the former expression in kidneys was examined by immunostaining.In biopsy tissues of ATN with severe histological features, CD147 induction was strikingly present in inflammatory cells such as macrophages and lymphocytes in the injured interstitium, but not in damaged tubules representing atrophy. Both plasma and urinary CD147 levels were strikingly increased in ATN patients; both values showed greater correlations with renal dysfunction compared to urinary l-FABP. In patients who had undergone open AAA surgery, urinary and plasma CD147 values in AKI patients were significantly higher than in non-AKI patients at post-operative day 1, similar to the profile of urinary l-FABP.CD147 was prominent in its ability to detect AKI and may allow the start of preemptive medication.