Identification of Stylosanthes guianensis varieties using molecular genetic analysis.
Identification of Stylosanthes guianensis varieties using molecular genetic analysis.
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DOI：
10.1093/aobpla/pls001
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发表时间：
2012
期刊：
影响因子：
2.9
通讯作者：
Souza AP
中科院分区：
文献类型：
--
作者：
Santos-Garcia MO;Karia CT;Resende RM;Chiari L;Vieira ML;Zucchi MI;Souza AP
利用分子遗传多样性和群体结构分析对柱花草植物分类进行了澄清。聚类为9组，每组主要由4个植物品种中的1个组成。柱花草的植物学分类存在争议，很少有研究利用分子标记对其进行分析。利用微卫星标记技术研究了桂树的遗传多样性和群体结构，并与现有的种下植物分类方法进行了比较。利用20个微卫星位点对150份巴西桂楠种质资料进行了分析。在STRUCTURE软件中实现了基于模型的贝叶斯方法，将接入分配到集群中。根据罗杰的遗传距离构建了一个树突图。每个位点的等位基因数为2 ~ 11个，平均为4.7个。观察到的（HO）杂合度为0 ~ 0.58（平均0.18），期望的（HE）杂合度为0.04 ~ 0.83（平均0.55）。在STRUCTURE中组装了9个类群，这些类群与根据遗传距离和分类品种推断的聚类一致。9组间GST均为0.46。观察到的低HO值和GST值与该物种估计的异交率（26%）一致。这些数据表明，植物品种之间和品种内部具有高度的遗传多样性，并表明基于微卫星的信息可以与经典分类学相结合，以阐明种下水平。
Molecular genetic diversity and population structure analysis were used to clarify the controversial botanical classification of Stylosanthes guianensis. The accessions were clustered in nine groups, each of which was mainly composed of only one of the four botanical varieties. The botanical classification of Stylosanthes guianensis is controversial, and few studies have used molecular markers to analyse this species. We used microsatellite markers to study the genetic diversity and population structure of S. guianensis and compare our results with the current infraspecific botanical classification. A representative sample from the S. guianensis Brazilian germplasm collection (150 accessions) was analysed using 20 microsatellite loci. A model-based Bayesian approach implemented in the software STRUCTURE was used to assign accessions into clusters. A dendrogram was constructed based on Roger's genetic distances. The number of alleles per locus varied from 2 to 11, with an average of 4.7. The observed (HO) and expected (HE) heterozygosity values varied from 0 to 0.58 (mean of 0.18) and from 0.04 to 0.83 (mean of 0.55), respectively. Nine groups were assembled in STRUCTURE, and these groups were consistent with clusters inferred from the genetic distances and taxonomic varieties described for S. guianensis. The GST among the nine groups was 0.46. The low HO and the GST values observed are in agreement with the outcrossing rate (26 %) estimated for this species. The data indicate a high genetic diversity among and within the botanical varieties and suggest that microsatellite-based information can be combined with classical taxonomy to elucidate infraspecific levels.