Investigation of the Origanum onites L. essential oil using the chorioallantoic membrane (CAM) assay
Investigation of the Origanum onites L. essential oil using the chorioallantoic membrane (CAM) assay
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DOI：
10.1021/jf034850k
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发表时间：
2004-01-28
影响因子：
6.1
通讯作者：
Baser, KHC
中科院分区：
文献类型：
--
作者：
Demirci, F;Paper, DH;Baser, KHC
对受精鸡蛋的绒毛尿囊膜的体内试验（CAM测定）是确定单个化合物或复合植物提取物的抗血管生成、促血管生成活性和毒性作用的当前方法。该方法用于测试少量天然化合物，以揭示与血管生成和炎症相关的各种作用模式和复杂机制。此外，还可以检测到可能的副作用，如膜刺激、毒性和所研究材料的抗凝特性。为了评价，通过水蒸馏牛至的地上部分获得的精油，一种常见的香料和药用植物，在绒毛尿囊膜（CAM）测定中测试其效果。采用气相色谱-质谱联用技术（GC-MS）分析了挥发油的组成。共鉴定出83种成分，占总油的99.1%。香芹酚、百里酚、对伞花烃和γ-萜品烯被发现为主要成分，并且在CAM测定中也被单独测试。沿着单萜香芹酚和百里酚，它们的甲基醚衍生物也被检测以比较它们的生理作用。在CAM测定中，在10-250 μ g/颗粒时，精油及其组分均未显示出任何显著的抗血管生成或抗血管生成特性。然而，精油的刺激作用与百里酚以剂量反应方式相关，高达10 μ g/粒，其中仍然显示刺激。
The in vivo test on the chorioallantoic membrane of the fertilized hen's egg (CAM assay) is a current method to determine antiangiogenic, antiinflammatory activity and toxic effects of individual compounds or complex plant extracts. The method is used for testing natural compounds in small amounts for revealing various modes of action and the complex mechanisms related to angiogenesis and inflammation. Furthermore, possible side effects Such as membrane irritation, toxic, and anticoagulant properties of the investigated material in question can be detected. For the evaluation, the essential oil obtained by hydrodistillation of the aerial parts of Origanum onites L., a common spice and medicinal plant, was tested for its effect in the chorioallantoic membrane (CAM) assay. The essential oil composition was revealed by means of gas chromatography-mass spectrometry (GC-MS). Eighty three components were identified, representing 99.1% of the total oil. Carvacrol, thymol, p-cymene, and gamma-terpinene were found as major components and were also individually tested in the CAM assay. Along with the monoterpenes carvacrol and thymol, their methyl ether derivatives were also examined for comparison of their physiological action. Neither the essential oil nor its components showed any pronounced antiinflammatory or antiangiogenic property in the CAM assay, at 10-250 mug/pellet. However, the irritant effect of the essential oil was linked to thymol in a dose-response fashion, up to 10 mug/pellet, where it was still showing irritation.