Regulation of hepatic enzymes and insulin levels in offspring of rat dams fed a reduced-protein diet
Regulation of hepatic enzymes and insulin levels in offspring of rat dams fed a reduced-protein diet
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DOI：
10.1152/ajpgi.1997.273.4.g899
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发表时间：
1997-10-01
影响因子：
4.5
通讯作者：
Hales, CN
中科院分区：
文献类型：
--
作者：
Desai, M;Byrne, CD;Hales, CN
我们假设，由于母亲营养不良而导致的早期发育不良造成的永久性变化，可能会因后代在以后的生活中营养过剩而加剧。为了验证这一假设，大鼠在胎儿和出生后的生活中接受了母体20%的蛋白质饮食或等卡路里的8%蛋白质饮食。所有仔鼠在6wk时断奶到实验室饲料上，大鼠接受实验室饲料或高适口性饮食(高脂肪、高热量和足够的蛋白质)，并在禁食48h后12wk进行研究。这种非常可口的饮食导致所有动物的体重增加和血浆胰岛素水平升高。饲喂低蛋白饲料的雄性仔鼠的血浆胰岛素浓度显著高于饲喂适量蛋白质日粮的雄性仔鼠，但雌性后代之间没有差异。蛋白质限制的大鼠DAMS子代体内的葡萄糖稳态的关键肝酶在成年后仍能对营养过剩作出反应。然而，在这些后代中，酶被调节在一个与对照组不同的“设定点”附近。
We have hypothesized that permanent changes caused by poor growth during early development due to maternal malnutrition may be exacerbated by overnutrition of offspring in later life. To test this hypothesis, rats were exposed to a maternal 20% protein diet or an isocaloric 8% protein diet during fetal and postnatal life. All offspring were weaned onto laboratory chow At 6 wk, rats were fed laboratory chow or a highly palatable diet (high fat and high calorie with adequate protein) and studied at 12 wk after a 48-h fast. The highly palatable diet resulted in excess weight gain and higher plasma insulin levels in all animals. Plasma insulin concentrations were significantly increased in male offspring of dams fed a reduced-protein diet compared with male offspring of dams fed an adequate-protein diet, but no differences were observed between the female offspring. The key hepatic enzymes of glucose homeostasis programmed in offspring of protein-restricted rat dams retained the ability to respond to overnutrition during adult Life. In these offspring, however, the enzymes were regulated around a ''set point'' that was different from that in the controls.