Polymorphic secreted kinases are key virulence factors in toxoplasmosis
Polymorphic secreted kinases are key virulence factors in toxoplasmosis
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DOI：
10.1126/science.1133690
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发表时间：
2006-12-15
期刊：
影响因子：
56.9
通讯作者：
Boothroyd, J. C.
中科院分区：
文献类型：
--
作者：
Saeij, J. P. J.;Boyle, J. P.;Boothroyd, J. C.
来自欧洲和北美的大多数已知弓形虫分离株属于三个克隆系，其毒力根据宿主而显著不同。为了确定相关基因，我们绘制了来自II型和III型菌株杂交的F-1后代的毒力图谱，并将其引入小鼠。五个毒力（VIR）位点，从而确定，其中两个，遗传互补表明，预测的蛋白激酶（ROP 18和ROP 16，分别）是关键分子。两者都是高变棒状体蛋白，在入侵时分泌到宿主细胞中。这些结果表明，分泌激酶独特的顶复门是至关重要的主机-病原体的相互作用。
The majority of known Toxoplasma gondii isolates from Europe and North America belong to three clonal lines that differ dramatically in their virulence, depending on the host. To identify the responsible genes, we mapped virulence in F-1 progeny derived from crosses between type II and type III strains, which we introduced into mice. Five virulence ( VIR) loci were thus identified, and for two of these, genetic complementation showed that a predicted protein kinase (ROP18 and ROP16, respectively) is the key molecule. Both are hypervariable rhoptry proteins that are secreted into the host cell upon invasion. These results suggest that secreted kinases unique to the Apicomplexa are crucial in the host-pathogen interaction.