THE C-TYPE CARBOHYDRATE-RECOGNITION DOMAIN (CRD) SUPERFAMILY
THE C-TYPE CARBOHYDRATE-RECOGNITION DOMAIN (CRD) SUPERFAMILY
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DOI：
10.1042/bst0220083
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发表时间：
1994-02-01
影响因子：
3.9
通讯作者：
DAY, AJ
中科院分区：
文献类型：
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作者：
DAY, AJ
C 型（CaL+ 依赖性）碳水化合物识别结构域 (CRD) 是一种蛋白质模块，存在于涉及多种功能的多种蛋白质超家族中（图 1）。这些功能是由以 Ca'+ 依赖性方式与碳水化合物结构特异性相互作用的一般特性介导的。 CRD 的长度从大约 11-5 到 130 个氨基酸不等，包含四个不变的半胱氨酸：二硫键连接的半胱氨酸1-半胱氨酸4 和半胱氨酸2-半胱氨酸3 [11（见图2）。一些 CRD 在 N 末端包含两个额外的二硫键半胱氨酸（包含四个半胱氨酸的 CRD 有时称为“短型 0”，而包含六个半胱氨酸的 CRD 则称为“长型 CRD”）。 CRD 具有主要基于疏水性残基的特征性共有序列，如图 2 所示。根据 CRD 的氨基酸序列比较，CRD 超家族的蛋白质可以细分为六个主要组 [11。 CRD超家族的成员如图1所示，相应的多序列比对如图2所示。可以看出，在任何特定的组中，蛋白质都具有相同的总体分子结构和高度相关的序列以及相关功能，如下所述。
The C-type (CaL+-dependent) carbohydrate recognition domain (CRD) is a protein module which is found in a diverse superfamily of proteins involved in a wide range of functions (Figure 1). These functions are mediated by the general property of interacting specifically with carbohydrate structures in a Ca’+-dependent manner. CRDs vary in length from about 11 5 to 130 amino acids and contain four invariant cysteines: disulphide-bonded cysteinelcysteine4 and cysteine2-cysteine3 [11 (see Figure 2). Some CRDs contain two additional disulphidebonded cysteines at the N-terminus (CRDs containing four cysteines are sometimes referred as ‘short-form 0 s’ and those containing six cysteines as ‘long-form CRDs’). CRDs have a characteristic consensus sequence based largely on hydrophobic residues as shown in Figure 2. The proteins of the CRD superfamily can be subdivided into six main groups on the basis of amino acid sequence comparisons of the CRDs [11. The members of the CRD superfamily are shown schematically in Figure 1 with a corresponding multiple sequence alignment in Figure 2. It can be seen that in any particular group the proteins all have the same overall molecular architecture and highly related sequences as well as related functions as will be discussed below.