Basal cell carcinoma and pilomatrixoma mirror human follicular embryogenesis as reflected by their differential expression patterns of SOX9 and β-catenin
Basal cell carcinoma and pilomatrixoma mirror human follicular embryogenesis as reflected by their differential expression patterns of SOX9 and β-catenin
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DOI：
10.1111/j.1365-2133.2010.09630.x
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发表时间：
2010-06-01
影响因子：
10.3
通讯作者：
Sellheyer, K.
中科院分区：
文献类型：
--
作者：
Krahl, D.;Sellheyer, K.
背景目前附件肿瘤的分类方案主要基于与成人皮肤结构的形态学和免疫表型相似性，然而，皮肤胚胎学与附件肿瘤组织发生之间的联系却被极大地忽视了。目的描述两种与毛囊稳态相关的蛋白质的表达模式方法采用免疫组化方法检测SOX 9和β-catenin在人皮肤胚胎发生过程中的表达，并与基底细胞癌（BCC）和毛母质瘤（pilomatrixoma）的表达进行比较。结果SOX 9和β-catenin在人毛囊胚胎发育过程中的表达模式与基底细胞癌和毛母质瘤在毛囊亚区的空间分布模式相一致。从发栓阶段开始，β-连环蛋白的核质免疫反应性仅限于新兴的基质（相当于pilomatrixoma），而SOX 9仅限于原始外根鞘（相当于BCC）。结论皮肤发育生物学的概念框架内验证了适当的免疫表型允许一个合理的分类附属器肿瘤。进一步扩展这种方法有可能修改目前的分类方案，使不仅BCC和毛母质瘤，但所有的附属器肿瘤可以进行逻辑分类。
Background The current classification schemes of adnexal tumours are predominantly based on morphological and immunophenotypical similarities to adult skin structures, whereas a link between the embryology of the skin and the histogenesis of adnexal tumours has been largely neglected.Objective To describe the expression patterns of two proteins with proven relevance for hair follicle homeostasis (SOX9 and beta-catenin) during human cutaneous embryogenesis and to compare the findings with their expression in basal cell carcinoma (BCC) and pilomatrixoma.Methods Immunohistochemical evaluation with monoclonal antibodies against SOX9 and beta-catenin was carried out in embryonic and adult human scalp skin, and BCC and pilomatrixoma samples.Results We found that the expression patterns of SOX9 and beta-catenin during human hair follicle embryogenesis mirror the patterns in BCC and pilomatrixoma in spatial distribution within the various follicular subcompartments. Beginning with the hair peg stage, nucleocytoplasmic immunoreactivity of beta-catenin is exclusively confined to the emerging matrix (comparable to pilomatrixoma), whereas SOX9 is restricted to the primordial outer root sheath (comparable to BCC).Conclusions An appropriate immunophenotyping validated within the conceptual framework of cutaneous developmental biology allows a logical classification of adnexal neoplasms. Expanding this approach further has the potential to revise the current classification schemes so that not only BCC and pilomatrixoma but all adnexal tumours can be categorized logically.