Temperature affects viral kinetics and vectorial capacity of Aedes aegypti mosquitoes co-infected with Mayaro and Dengue viruses.
Temperature affects viral kinetics and vectorial capacity of Aedes aegypti mosquitoes co-infected with Mayaro and Dengue viruses.
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温度影响与马亚罗病毒和登革热病毒共感染的埃及伊蚊的病毒动力学和传播能力。
DOI：
10.1101/2023.05.17.541186
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发表时间：
2023
期刊：
影响因子：
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通讯作者：
Rasgon,JasonL
中科院分区：
文献类型：
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作者：
Terradas,Gerard;Manzano-Alvarez,Jaime;Vanalli,Chiara;Werling,Kristine;Cattadori,IsabellaM;Rasgon,JasonL
背景全球气温升高和不可预测的极端气候导致了病媒传播疾病的传播。埃及伊蚊是多种虫媒病毒的主要载体，对人类健康产生负面影响，主要发生在世界上社会经济水平较低的地区。这些病毒在人类的共循环和共感染已越来越多地报道，然而，如何载体有助于这一惊人的trends.MethodsHere，我们研究单一和共同感染的马亚罗病毒（D株，甲病毒）和登革病毒（血清型2，黄病毒）在Ae。的aegyptiadults和细胞系在两个恒定的温度下，温和（27 °C）和热（32 °C），量化载体的能力和温度对感染，传播和传播的影响，包括对两种病毒之间的相互作用的程度。登革病毒在两种温度下在成年蚊子中快速复制，在共感染蚊子中滴度倾向于更高，并且在所有条件下，蚊子死亡率在较高温度下更严重。对于登革，以及在较小程度上对于Mayaro，媒介能力和媒介能力在较热的温度下在共同感染中比在单一感染中更高，并且对于Mayaro在较早的时间点（感染后7天比14天）更明显。温度依赖性的表型在体外证实了更快的细胞感染和初始复制在较高的温度为登革热，但不是Mayaro virus.ConclusionsOur研究表明，这两种病毒的对比动力学可能与其内在的热要求，甲病毒茁壮成长更好地在较低的温度相比，黄病毒。然而，还需要更多的研究来阐明在不同的温度条件下，包括在更自然的温度设置下，共同感染的作用。
BackgroundIncreasing global temperatures and unpredictable climatic extremes have contributed to the spread of vector-borne diseases. The mosquitoAedes aegyptiis the main vector of multiple arboviruses that negatively impact human health, mostly in low socioeconomic areas of the world. Co-circulation and co-infection of these viruses in humans have been increasingly reported; however, how vectors contribute to this alarming trend remains unclear.MethodsHere, we examine single and co-infection of Mayaro virus (D strain,Alphavirus) and dengue virus (serotype 2,Flavivirus) inAe. aegyptiadults and cell lines at two constant temperatures, moderate (27 °C) and hot (32 °C), to quantify vector competence and the effect of temperature on infection, dissemination and transmission, including on the degree of interaction between the two viruses.ResultsBoth viruses were primarily affected by temperature but there was a partial interaction with co-infection. Dengue virus quickly replicates in adult mosquitoes with a tendency for higher titers in co-infected mosquitoes at both temperatures, and mosquito mortality was more severe at higher temperatures in all conditions. For dengue, and to a lesser extent Mayaro, vector competence and vectorial capacity were higher at hotter temperature in co- vs. single infections and was more evident at earlier time points (7 vs. 14 days post infection) for Mayaro. The temperature-dependent phenotype was confirmed in vitro by faster cellular infection and initial replication at higher temperatures for dengue but not for Mayaro virus.ConclusionsOur study suggests that contrasting kinetics of the two viruses could be related to their intrinsic thermal requirements, where alphaviruses thrive better at lower temperatures compared to flaviviruses. However, more studies are necessary to clarify the role of co-infection at different temperature regimes, including under more natural temperature settings.Graphical Abstract