Dynein light chain LC8 is a dimerization hub essential in diverse protein networks
Dynein light chain LC8 is a dimerization hub essential in diverse protein networks
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DOI：
10.1021/bi701995m
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发表时间：
2008-01-15
期刊：
影响因子：
2.9
通讯作者：
Barbar, Elisar
中科院分区：
文献类型：
--
作者：
Barbar, Elisar
活细胞内的活动依赖于蛋白质网络的集体活动，有时被称为“相互作用组”。在这些网络中，大多数蛋白质与很少的伙伴相互作用，而一小部分称为枢纽的蛋白质参与大量的相互作用，并在组织这些相互作用组中发挥核心作用。LC8最初是作为微管分子动力蛋白的重要组成部分被发现的，因此它参与了一些基本过程，包括逆行囊泡运输、纤毛/鞭毛运动和细胞分裂。最近，越来越多的证据表明，LC8也与一些与动力蛋白或微管转运没有明确联系的蛋白相互作用，包括一些在细胞凋亡、病毒发病、酶调节和肾脏发育中起作用的蛋白。在这里，我们介绍了LC8是多种蛋白质网络中必不可少的枢纽蛋白，其作为动力蛋白轻链的功能只是众多蛋白中的一种。我们进一步提出，LC8在各种系统中的关键调节作用是由于它能够促进部分紊乱蛋白的二聚化。
The operations within a living cell depend on the collective activity of networks of proteins, sometimes termed "interactomes". Within these networks, most proteins interact with few partners, while a small proportion of proteins, called hubs, participate in a large number of interactions and play a central role in organizing these interactomes. LC8 was first discovered as an essential component of the microtubule-based molecular inotor dynein and as Such is involved in fundamental processes, including retrograde vesicular trafficking, ciliary/flagellar motility, and cell division. More recently, evidence has accumulated that LC8 also interacts with proteins that are not clearly connected with dynein or rnicrotubule-based transport, including some with roles in apoptosis, viral pathogenesis, enzyme regulation, and kidney development. Here, we introduce the idea that LC8 is a hub protein essential in diverse protein networks, and its function as a dynein light chain is but one of many. We further propose that the crucial regulatory roles of LC8 in various systems are due to its ability to promote dimerization of partially disordered proteins.